Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/111840
DC FieldValue
dc.titleCytotoxicity and ADP-ribosylating activity of the mosquitocidal toxin from Bacillus sphaericus SSII-1: Possible roles of the 27- and 70-kilodalton peptides
dc.contributor.authorThanabalu, T.
dc.contributor.authorBerry, C.
dc.contributor.authorHindley, J.
dc.date.accessioned2014-11-28T02:50:16Z
dc.date.available2014-11-28T02:50:16Z
dc.date.issued1993
dc.identifier.citationThanabalu, T.,Berry, C.,Hindley, J. (1993). Cytotoxicity and ADP-ribosylating activity of the mosquitocidal toxin from Bacillus sphaericus SSII-1: Possible roles of the 27- and 70-kilodalton peptides. Journal of Bacteriology 175 (8) : 2314-2320. ScholarBank@NUS Repository.
dc.identifier.issn00219193
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/111840
dc.description.abstractClones expressing regions of the 100-kDa Bacillus sphaericus SSII-1 mosquitocidal toxin (Mtx) as fusion proteins with glutathione S-transferase were constructed, and the toxin-derived peptides were purified. The in vitro ADP-ribosylation activities of these peptides and their effects on larvae and cells in culture were studied. Mtx25 (amino acids 30 to 493) was found to ADP-ribosylate two proteins with molecular masses of 38 and 42 kDa, respectively, in Culex quinquefasciatus (G7) cell extracts, in addition to ADP-ribosylating itself. Mtx21 (amino acids 30 to 870; or a combination of Mtx25 and Mtx26 (amino acids 259 to 870) caused mortality in C. quinquefasciatus larvae. Mtx25, Mtx26, or Mtx24 (amino acids 30 to 276) alone and Mtx24 in combination with Mtx26 were not toxic to larvae. Mtx21 and Mtx26 produced marked morphological changes in G7 cells and to a lesser extent in Aedes aegypti cells but had no effect on Anopheles gambiae or HeLa cells. Thus, a domain in the N-terminal region of the Mtx protein is sufficient for ADP-ribosylation of C. quinquefasciatus cell protein, and a domain in the C- terminal region is sufficient for toxicity to cultured C. quinquefasciatus cells; however, both regions are necessary for toxicity to mosquito larvae.
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentINSTITUTE OF MOLECULAR & CELL BIOLOGY
dc.description.sourcetitleJournal of Bacteriology
dc.description.volume175
dc.description.issue8
dc.description.page2314-2320
dc.description.codenJOBAA
dc.identifier.isiutNOT_IN_WOS
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

Page view(s)

92
checked on Aug 4, 2022

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.