Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/111780
Title: An analysis of the genes encoding the 51.4- and 41.9-kDa toxins of Bacillus sphaericus 2297 by deletion mutagenesis: the construction of fusion proteins
Authors: Oei, C. 
Hindley, J. 
Berry, C. 
Keywords: Bacillus sphaericus
Deletion mutants
Fusion proteins
Mosquitocidal toxins
Issue Date: Nov-1990
Citation: Oei, C.,Hindley, J.,Berry, C. (1990-11). An analysis of the genes encoding the 51.4- and 41.9-kDa toxins of Bacillus sphaericus 2297 by deletion mutagenesis: the construction of fusion proteins. FEMS Microbiology Letters 72 (3) : 265-273. ScholarBank@NUS Repository.
Abstract: A series of deletion mutants have been constructed, in which varying numbers of amino acids have been deleted from both the N- and C-termini of both the 51.4- and 41.9-kDa toxins of Bacillus sphaericus. The results show that between 34-39 and 52-54 amino acids respectively at the N- and C-termini of the 51.4-kDa protein, are not essential for toxicity. In the case of the 41.9-kDa protein, the removal of only 7 amino acids from the C-terminus abolishes toxicity whilst at least 17 amino acids can be deleted from the N-terminus without loss of toxicity. A fusion protein with the 51.4-kDa derived sequence N-terminal to the 41.9-kDa sequence yielded a protein of Mr 87 kDa which was not toxic by itself. When supplemented with cells expressing only the 51.4-kDa protein, toxicity was restored. In contrast, another fusion protein, in which the gene order was reversed, was shown to be fully active in toxicity assays. © 1990.
Source Title: FEMS Microbiology Letters
URI: http://scholarbank.nus.edu.sg/handle/10635/111780
ISSN: 03781097
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

91
checked on Jun 23, 2022

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.