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Title: A cDNA clone encoding Brassica calmodulin
Authors: Chye, M.-L. 
Liu, C.-M.
Tan, C.-T. 
Keywords: calcium-binding protein
in situ hybridisation
root tip
shoot apical meristem
Issue Date: Jan-1995
Citation: Chye, M.-L., Liu, C.-M., Tan, C.-T. (1995-01). A cDNA clone encoding Brassica calmodulin. Plant Molecular Biology 27 (2) : 419-423. ScholarBank@NUS Repository.
Abstract: A 834 bp cDNA encoding calmodulin (CaM) has been isolated from Brassica juncea. On Northern analysis this cDNA hybridises this cDNA to mRNAs of about 0.9 kb in leaf, silique and peduncle. Genomic Southern analysis indicates the presence of a CaM multigene family in Brassica juncea. Comparison of the predicted amino acid sequence of Brassica CaM with that of Arabidopsis CaM ACaM-2 and ACaM-3 showed 100% homology, which is not unusual, since both plants belong to the family Cruciferae. In situ hybridisation studies on Brassica seedlings using a digoxigenin-labelled RNA probe showed that high levels of CaM mRNA were detected in the leaf primordia and the shoot apical meristem, and to a lesser degree, in the zone of root elongation of the root tip. The occurrence of a higher rate of cell division and growth in these regions than its surrounding tissue may possibly be related to higher levels of CaM mRNA. © 1995 Kluwer Academic Publishers.
Source Title: Plant Molecular Biology
ISSN: 01674412
DOI: 10.1007/BF00020195
Appears in Collections:Staff Publications

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