Please use this identifier to cite or link to this item:
Title: Continuous epitopes of human lymphotoxin and their topographies
Authors: Yao, Z.-J. 
Kao, M.C.C. 
Loh, K.-C. 
Chung, M.C.M.
Issue Date: 1994
Citation: Yao, Z.-J.,Kao, M.C.C.,Loh, K.-C.,Chung, M.C.M. (1994). Continuous epitopes of human lymphotoxin and their topographies. Biochemistry and Molecular Biology International 32 (5) : 951-959. ScholarBank@NUS Repository.
Abstract: Human lymphotoxin (hLT or TNF-β) is a lymphokine that is structurally and functionally related to tumor necrosis factor alpha (TNF-α). The continuous epitopes of hLT were located by examining the cross-reaction between rabbit anti-hLT antibody and peptides derived from proteolytic digestion and chemical synthesis. Three antigenic sites, corresponding to residues 40-48, 83-94 and 139-147, of the protein sequence, were located by this approach. Since residues 49-57 also exhibited trace antigenicity, but residues 45-52 displayed no reaction, the whole peptide fragment consisting of residues 40-57 might be necessary for antigenicity. A comparison of the antigenic determinants with the loop structures obtained from X-ray crystallographic studies of hLT showed that all of the epitopes are found on or adjacent to functionally important domains.
Source Title: Biochemistry and Molecular Biology International
ISSN: 10399712
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

checked on Sep 22, 2022

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.