Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0165-2478(98)00029-7
Title: An immunogenic epitope of Chlamydia pneumoniae from a random phage display peptide library is reactive with both monoclonal antibody and patients sera
Authors: Naidu, B.R.
Ngeow, Y.-F.
Wang, L.-F.
Chan, L. 
Yao, Z.-J. 
Pang, T.
Keywords: C. pneumoniae
Epitope
Phage display
Random peptide
Issue Date: Jun-1998
Citation: Naidu, B.R., Ngeow, Y.-F., Wang, L.-F., Chan, L., Yao, Z.-J., Pang, T. (1998-06). An immunogenic epitope of Chlamydia pneumoniae from a random phage display peptide library is reactive with both monoclonal antibody and patients sera. Immunology Letters 62 (2) : 111-115. ScholarBank@NUS Repository. https://doi.org/10.1016/S0165-2478(98)00029-7
Abstract: Random 15-mer peptides displayed on filamentous phages were screened in binding studies using a Chlamydia pneumoniae-specific monoclonal antibody (RR-402) and affinity-purified, polyclonal sera from patients seropositive for C. pneumoniae infections by the microimmunofluorescence (MIF) test. One 15-mer epitope, epitope Cpn15A (LASLCNPKPSDAPVT) was identified in both the monoclonal and polyclonal screenings, and showed higher ELISA reactivity with C. pneumoniae MIF-positive sera compared to patients with other chlamydial infections, non-chlamydial respiratory infections and normal healthy sera (MIF-negative). Interestingly, epitope Cpn15A also showed significant (52%) amino acid sequence homology to the 56 kDa type-specific antigen of Rickettsia tsutsugamushi, a protein implicated in the virulence of this organism.
Source Title: Immunology Letters
URI: http://scholarbank.nus.edu.sg/handle/10635/111033
ISSN: 01652478
DOI: 10.1016/S0165-2478(98)00029-7
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