Please use this identifier to cite or link to this item: https://doi.org/10.1107/S174430911103836X
Title: Cloning, expression, purification, crystallization and preliminary X-ray diffraction studies of a major group 7 allergen, der f 7, from the dust mite Dermatophagoides farinae
Authors: Tan, K.W.
Kumar, S. 
Chew, F.T. 
Mok, Y.K. 
Keywords: Der f 7
Dermatophagoides farinae
group 7 allergens
Issue Date: Dec-2011
Citation: Tan, K.W., Kumar, S., Chew, F.T., Mok, Y.K. (2011-12). Cloning, expression, purification, crystallization and preliminary X-ray diffraction studies of a major group 7 allergen, der f 7, from the dust mite Dermatophagoides farinae. Acta Crystallographica Section F: Structural Biology and Crystallization Communications 67 (12) : 1612-1615. ScholarBank@NUS Repository. https://doi.org/10.1107/S174430911103836X
Abstract: Der f 7 is a major group 7 allergen from the dust mite Dermatophagoides farinae that shows 86% sequence identity to the homologous allergen Der p 7 from D. pteronyssinus. Der f 7 was successfully overexpressed in an Escherichia coli expression system and purified to homogeneity using Ni-NTA affinity and size-exclusion column chromatography. SeMet-labelled Der f 7 was crystallized by the hanging-drop vapour-diffusion method using a reservoir solution consisting of 0.1 M bis-tris pH 7.4 and 28% polyethylene glycol monomethyl ether 2000 at 293 K. X-ray diffraction data were collected to 2.24 Å resolution using synchrotron radiation. The crystals belonged to the orthorhombic system, space group P2 12 12 1, with unit-cell parameters a = 50.19, b = 58.67, c = 123.81 Å. Based on the estimated Matthews coefficient (2.16 Å 3 Da -1), two molecules of Der f 7 could be present in the asymmetric unit of the crystal lattice. © 2011 International Union of Crystallography. All rights reserved.
Source Title: Acta Crystallographica Section F: Structural Biology and Crystallization Communications
URI: http://scholarbank.nus.edu.sg/handle/10635/111000
ISSN: 17443091
DOI: 10.1107/S174430911103836X
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