Please use this identifier to cite or link to this item:
|Title:||Cloning, expression, purification, crystallization and preliminary X-ray diffraction studies of a major group 7 allergen, der f 7, from the dust mite Dermatophagoides farinae||Authors:||Tan, K.W.
|Keywords:||Der f 7
group 7 allergens
|Issue Date:||Dec-2011||Citation:||Tan, K.W., Kumar, S., Chew, F.T., Mok, Y.K. (2011-12). Cloning, expression, purification, crystallization and preliminary X-ray diffraction studies of a major group 7 allergen, der f 7, from the dust mite Dermatophagoides farinae. Acta Crystallographica Section F: Structural Biology and Crystallization Communications 67 (12) : 1612-1615. ScholarBank@NUS Repository. https://doi.org/10.1107/S174430911103836X||Abstract:||Der f 7 is a major group 7 allergen from the dust mite Dermatophagoides farinae that shows 86% sequence identity to the homologous allergen Der p 7 from D. pteronyssinus. Der f 7 was successfully overexpressed in an Escherichia coli expression system and purified to homogeneity using Ni-NTA affinity and size-exclusion column chromatography. SeMet-labelled Der f 7 was crystallized by the hanging-drop vapour-diffusion method using a reservoir solution consisting of 0.1 M bis-tris pH 7.4 and 28% polyethylene glycol monomethyl ether 2000 at 293 K. X-ray diffraction data were collected to 2.24 Å resolution using synchrotron radiation. The crystals belonged to the orthorhombic system, space group P2 12 12 1, with unit-cell parameters a = 50.19, b = 58.67, c = 123.81 Å. Based on the estimated Matthews coefficient (2.16 Å 3 Da -1), two molecules of Der f 7 could be present in the asymmetric unit of the crystal lattice. © 2011 International Union of Crystallography. All rights reserved.||Source Title:||Acta Crystallographica Section F: Structural Biology and Crystallization Communications||URI:||http://scholarbank.nus.edu.sg/handle/10635/111000||ISSN:||17443091||DOI:||10.1107/S174430911103836X|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Oct 14, 2019
WEB OF SCIENCETM
checked on Oct 7, 2019
checked on Oct 11, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.