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Title: Production of 7-deoxy-okadaic acid by a new caledonian strain of Prorocentrum Lima (Dinophyceae)
Authors: Holmes, M.J. 
Lee, F.C. 
Khoo, H.W. 
Teo, S.L.M. 
Keywords: 7-deoxy-okadaic acid
Diarrhetic shellfish poisoning
Okadaic acid
Prorocentrum lima
Issue Date: 2001
Citation: Holmes, M.J., Lee, F.C., Khoo, H.W., Teo, S.L.M. (2001). Production of 7-deoxy-okadaic acid by a new caledonian strain of Prorocentrum Lima (Dinophyceae). Journal of Phycology 37 (2) : 280-288. ScholarBank@NUS Repository.
Abstract: 7-Deoxy-okadaic acid and okadaic acid were identified as the major diarrhetic shellfish poisoning (DSP) toxins produced by a New Caledonian strain of Prorocentrum lima Ehrenberg. Dinophysistoxin-1 was not produced by this strain. The cellular concentrations of 7-deoxy-okadaic acid were about one tenth that of okadaic acid and were maximal (∼1.4 pg cell-1) during the stationary growth phase of batch culture. Autolytic hydrolysis of cell extracts did not increase the concentrations of 7-deoxy-okadaic acid, whereas okadaic acid production increased more than 4-fold, indicating that 7-deoxy-okadaic acid, unlike okadaic acid, is not directly derived from large sulfated precursors. 7-Deoxy-okadaic acid could be detected by liquid chromatography-selected reaction monitoring mass spectrometry, HPLC-fluorescence detection after derivatization with 9-anthryldiazomethane (ADAM), and inhibition of protein phosphatases. The solvent washes currently used for solid-phase clean-up of ADAM-derivatized DSP samples elute derivatized 7-deoxy-okadaic acid, indicating that the current sample clean-up protocol for HPLC-fluorescence detection would miss any contamination by this toxin.
Source Title: Journal of Phycology
ISSN: 00223646
DOI: 10.1046/j.1529-8817.2001.037002280.x
Appears in Collections:Staff Publications

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