Please use this identifier to cite or link to this item:
https://doi.org/10.1016/S0166-0934(02)00212-4
DC Field | Value | |
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dc.title | Production and characterization of monoclonal antibodies to a grouper iridovirus | |
dc.contributor.author | Shi, C. | |
dc.contributor.author | Qin, Q.W. | |
dc.contributor.author | Gin, K.Y.H. | |
dc.contributor.author | Lam, T.J. | |
dc.date.accessioned | 2014-11-26T10:26:51Z | |
dc.date.available | 2014-11-26T10:26:51Z | |
dc.date.issued | 2003-02 | |
dc.identifier.citation | Shi, C., Qin, Q.W., Gin, K.Y.H., Lam, T.J. (2003-02). Production and characterization of monoclonal antibodies to a grouper iridovirus. Journal of Virological Methods 107 (2) : 147-154. ScholarBank@NUS Repository. https://doi.org/10.1016/S0166-0934(02)00212-4 | |
dc.identifier.issn | 01660934 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/110893 | |
dc.description.abstract | A panel of six monoclonal antibodies (mAbs) against a grouper iridovirus (SGIV) were produced by immunization of Balb/c mice with purified virus preparations. Isotyping test revealed all the mAbs were IgG1. None of the mAbs possessed the ability to neutralize SGIV in cell cultures. Western blot showed that 4 mAbs reacted with 2 SGIV proteins at molecular mass of approximately 100 and 117 kDa in gradient-purified virus. Immunofluorescent studies showed that the two specific viral proteins VP100 and VP117 were localized within virus assembly sites in the cytoplasm of SGIV-infected grouper cells (GP). Fractionations of the iridovirus in a 20-60% sucrose gradient were detected successfully by all the six mAbs using immunodot blot. An antigen-capture enzyme-linked immunosorbent assay (ELISA) system, based on the use of mAb 7E11 for capture and a rabbit polyclonal antibody to SGIV for detection was developed. SGIV antigen was detected in gradient-purified virus and virus-infected grouper blood. These novel mAbs will facilitate the development of more specific and standardized diagnostic techniques for marine fish iridovirus. © 2002 Elsevier Science B.V. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0166-0934(02)00212-4 | |
dc.source | Scopus | |
dc.subject | Epinephelus spp. | |
dc.subject | Grouper | |
dc.subject | Iridovirus | |
dc.subject | Monoclonal antibodies | |
dc.type | Article | |
dc.contributor.department | TROPICAL MARINE SCIENCE INSTITUTE | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.description.doi | 10.1016/S0166-0934(02)00212-4 | |
dc.description.sourcetitle | Journal of Virological Methods | |
dc.description.volume | 107 | |
dc.description.issue | 2 | |
dc.description.page | 147-154 | |
dc.description.coden | JVMED | |
dc.identifier.isiut | 000180490500005 | |
Appears in Collections: | Staff Publications |
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