Please use this identifier to cite or link to this item:
Title: Production and characterization of monoclonal antibodies to a grouper iridovirus
Authors: Shi, C.
Qin, Q.W. 
Gin, K.Y.H.
Lam, T.J. 
Keywords: Epinephelus spp.
Monoclonal antibodies
Issue Date: Feb-2003
Citation: Shi, C., Qin, Q.W., Gin, K.Y.H., Lam, T.J. (2003-02). Production and characterization of monoclonal antibodies to a grouper iridovirus. Journal of Virological Methods 107 (2) : 147-154. ScholarBank@NUS Repository.
Abstract: A panel of six monoclonal antibodies (mAbs) against a grouper iridovirus (SGIV) were produced by immunization of Balb/c mice with purified virus preparations. Isotyping test revealed all the mAbs were IgG1. None of the mAbs possessed the ability to neutralize SGIV in cell cultures. Western blot showed that 4 mAbs reacted with 2 SGIV proteins at molecular mass of approximately 100 and 117 kDa in gradient-purified virus. Immunofluorescent studies showed that the two specific viral proteins VP100 and VP117 were localized within virus assembly sites in the cytoplasm of SGIV-infected grouper cells (GP). Fractionations of the iridovirus in a 20-60% sucrose gradient were detected successfully by all the six mAbs using immunodot blot. An antigen-capture enzyme-linked immunosorbent assay (ELISA) system, based on the use of mAb 7E11 for capture and a rabbit polyclonal antibody to SGIV for detection was developed. SGIV antigen was detected in gradient-purified virus and virus-infected grouper blood. These novel mAbs will facilitate the development of more specific and standardized diagnostic techniques for marine fish iridovirus. © 2002 Elsevier Science B.V. All rights reserved.
Source Title: Journal of Virological Methods
ISSN: 01660934
DOI: 10.1016/S0166-0934(02)00212-4
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Aug 8, 2020


checked on Jul 31, 2020

Page view(s)

checked on Aug 1, 2020

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.