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|Title:||Production and characterization of monoclonal antibodies to a grouper iridovirus||Authors:||Shi, C.
|Issue Date:||Feb-2003||Citation:||Shi, C., Qin, Q.W., Gin, K.Y.H., Lam, T.J. (2003-02). Production and characterization of monoclonal antibodies to a grouper iridovirus. Journal of Virological Methods 107 (2) : 147-154. ScholarBank@NUS Repository. https://doi.org/10.1016/S0166-0934(02)00212-4||Abstract:||A panel of six monoclonal antibodies (mAbs) against a grouper iridovirus (SGIV) were produced by immunization of Balb/c mice with purified virus preparations. Isotyping test revealed all the mAbs were IgG1. None of the mAbs possessed the ability to neutralize SGIV in cell cultures. Western blot showed that 4 mAbs reacted with 2 SGIV proteins at molecular mass of approximately 100 and 117 kDa in gradient-purified virus. Immunofluorescent studies showed that the two specific viral proteins VP100 and VP117 were localized within virus assembly sites in the cytoplasm of SGIV-infected grouper cells (GP). Fractionations of the iridovirus in a 20-60% sucrose gradient were detected successfully by all the six mAbs using immunodot blot. An antigen-capture enzyme-linked immunosorbent assay (ELISA) system, based on the use of mAb 7E11 for capture and a rabbit polyclonal antibody to SGIV for detection was developed. SGIV antigen was detected in gradient-purified virus and virus-infected grouper blood. These novel mAbs will facilitate the development of more specific and standardized diagnostic techniques for marine fish iridovirus. © 2002 Elsevier Science B.V. All rights reserved.||Source Title:||Journal of Virological Methods||URI:||http://scholarbank.nus.edu.sg/handle/10635/110893||ISSN:||01660934||DOI:||10.1016/S0166-0934(02)00212-4|
|Appears in Collections:||Staff Publications|
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