Please use this identifier to cite or link to this item:
|Title:||Prophylactic uses of integrin CD18-βA peptide in a murine polymicrobial peritonitis model||Authors:||Wong, K.-F.
|Issue Date:||7-Jun-2010||Citation:||Wong, K.-F., Wo, J., Ho, D., Poon, R.T., Casasnovas, J.M., Luk, J.M. (2010-06-07). Prophylactic uses of integrin CD18-βA peptide in a murine polymicrobial peritonitis model. World Journal of Gastroenterology 16 (21) : 2648-2656. ScholarBank@NUS Repository. https://doi.org/10.3748/wjg.v16.i21.2648||Abstract:||Aim: To evaluate the prophylactic properties of integrin CD18-βA peptide in a murine model of abdominal polymicrobial peritonitis and sepsis. Methods: Bacterial sepsis was induced in Institute of Cancer Research (ICR) mice by cecal ligation and puncture (CLP) surgery. Inflicted mice were then injected with either sterile saline or CD18-βA peptide intraperi-toneally at 2 h after surgery, and were sacrifced at 12 and 24 h after surgery. Blood samples were immediately collected, and analyzed for endotoxin activity and tumor necrosis factor (TNF)-α and interleukin (IL)-6. Lungs and liver were studied for CD45+ leukocyte and CD3 mRNA content. Pulmonary expression of intercellular adhesion molecule (ICAM)-1, vascular cell adhesion molecule (VCAM) and E-selectin was also determined. Results: Intraperitoneal injection of CD18-βA peptide signifcantly suppressed circulating endotoxin activity (P < 0.01) at 24 h, as well as serum levels of TNF-α (P < 0.05 at 12 and 24 h) and IL-6 (P < 0.01 at 12 h, P < 0.05 at 24 h) in CLP-inflicted mice. CD18-βA peptide also abrogated leukocyte infiltration into liver and lungs as unveiled by reduced CD45+ leukocyte and CD3 mRNA contents. Furthermore, the peptide significantly reduced pulmonary expression of VCAM (P < 0.01 at 12 h, P < 0.001 at 24 h), E-selectin (P < 0.01 at 12 and 24 h), and ICAM-1 (P < 0.01 at 12 h, P < 0.001 at 24 h). These actions of CD18-βA peptide collectively protected septic mice against lethality (P < 0.01). Conclusion: CD18-βA peptide is a potent endotoxin antagonist that can protect surgical patients against sepsis-associated lethality. © 2010 Baishideng. All rights reserved.||Source Title:||World Journal of Gastroenterology||URI:||http://scholarbank.nus.edu.sg/handle/10635/110771||ISSN:||10079327||DOI:||10.3748/wjg.v16.i21.2648|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jul 3, 2020
WEB OF SCIENCETM
checked on Jul 3, 2020
checked on Jun 27, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.