Please use this identifier to cite or link to this item:
|Title:||Akt-Induced Phosphorylation of N-CoR at Serine 1450 Contributes to Its Misfolded Conformational Dependent Loss (MCDL) in Acute Myeloid Leukemia of the M5 Subtype||Authors:||Nin, D.S.
|Issue Date:||5-Aug-2013||Citation:||Nin, D.S., Ali, A.B., Okumura, K., Asou, N., Chen, C.-S., Chng, W.J., Khan, M. (2013-08-05). Akt-Induced Phosphorylation of N-CoR at Serine 1450 Contributes to Its Misfolded Conformational Dependent Loss (MCDL) in Acute Myeloid Leukemia of the M5 Subtype. PLoS ONE 8 (8) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0070891||Abstract:||The nuclear receptor co-repressor (N-CoR) is a key component of the generic co-repressor complex that plays an important role in the control of cellular growth and differentiation. As shown by us recently, the growth suppressive function of N-CoR largely relies on its capacity to repress Flt3, a key regulator of cellular gorwth during normal and malignant hematopoesis. We further demonstrated how de-repression of Flt3 due to the misfolded conformation dependent loss (MCDL) of N-CoR contributed to malignant growth in acute myeloid leukemia (AML). However, the molecular mechanism underlying the MCDL of N-CoR and its implication in AML pathogenesis is not fully understood. Here, we report that Akt-induced phosphorylation of N-CoR at the consensus Akt motif is crucial for its misfolding and subsequent loss in AML (AML-M5). N-CoR displayed significantly higher level of serine specific phosphorylation in almost all AML-M5 derived cells and was subjected to processing by AML-M5 specific aberrant protease activity. To identify the kinase linked to N-CoR phosphorylation, a library of activated kinases was screened with the extracts of AML cells; leading to the identification of Akt as the putative kinase linked to N-CoR phosphorylation. Consistent with this finding, a constitutively active Akt consistently phosphorylated N-CoR leading to its misfolding; while the therapeutic and genetic ablation of Akt largely abrogated the MCDL of N-CoR in AML-M5 cells. Site directed mutagenic analysis of N-CoR identified serine 1450 as the crucial residue whose phosphorylation by Akt was essential for the misfolding and loss of N-CoR protein. Moreover, Akt-induced phosphorylation of N-CoR contributed to the de-repression of Flt3, suggesting a cross talk between Akt signaling and N-CoR misfolding pathway in the pathogenesis of AML-M5. The N-CoR misfolding pathway could be the common downstream thread of pleiotropic Akt signaling activated by various oncogenic insults in some subtypes of leukemia and solid tumors. © 2013 Nin et al.||Source Title:||PLoS ONE||URI:||http://scholarbank.nus.edu.sg/handle/10635/110721||ISSN:||19326203||DOI:||10.1371/journal.pone.0070891|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
|2013_Akt-Induced_Phosphorylation_of_N-CoR_at-published.PDF||1.27 MB||Adobe PDF|
checked on Jul 16, 2019
WEB OF SCIENCETM
checked on Jul 9, 2019
checked on Jul 5, 2019
checked on Jul 5, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.