Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.chroma.2013.08.016
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dc.titleRapid and sensitive profiling of tear wax ester species using high performance liquid chromatography coupled with tandem mass spectrometry
dc.contributor.authorLam, S.M.
dc.contributor.authorTong, L.
dc.contributor.authorReux, B.
dc.contributor.authorLear, M.J.
dc.contributor.authorWenk, M.R.
dc.contributor.authorShui, G.
dc.date.accessioned2014-11-26T09:05:10Z
dc.date.available2014-11-26T09:05:10Z
dc.date.issued2013-09-20
dc.identifier.citationLam, S.M., Tong, L., Reux, B., Lear, M.J., Wenk, M.R., Shui, G. (2013-09-20). Rapid and sensitive profiling of tear wax ester species using high performance liquid chromatography coupled with tandem mass spectrometry. Journal of Chromatography A 1308 : 166-171. ScholarBank@NUS Repository. https://doi.org/10.1016/j.chroma.2013.08.016
dc.identifier.issn00219673
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/110622
dc.description.abstractA rapid and sensitive method was developed for quantitative profiling of wax esters (WEs) in human tear lipidome. Individual WE species was separated by liquid chromatography and detected by electrospray ionisation mass spectrometry using specific multiple reaction monitoring (MRM) scanning. Palmitoyl palmitate and in-house synthesized wax esters 13C18:1(oleic acid-1,2,3,7,8,9,10-13C7)C26:0 were used as internal standards for quantitation of WEs containing saturated and unsaturated fatty acids (FA), respectively. The limit of detection was approximately 70nmol/L. The linearity range of the liquid chromatography (LC)-MRM detection for WEs was about three orders of magnitude. Quantitative analyses of 141 individual WE in the human tear lipidome demonstrated that species comprising FA18:1 and FA16:1 each accounted for 47.7% and 24.0% (molar%) of total WE, while fatty alcohols in WEs of human tears ranged from 17 carbons to 32 carbons with predominant species represented by C24, C25 and C26. © 2013 Elsevier B.V.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.chroma.2013.08.016
dc.sourceScopus
dc.subjectMass spectrometry
dc.subjectMultiple reaction monitoring (MRM)
dc.subjectTear
dc.subjectWax esters
dc.typeArticle
dc.contributor.departmentCHEMISTRY
dc.contributor.departmentDUKE-NUS GRADUATE MEDICAL SCHOOL S'PORE
dc.description.doi10.1016/j.chroma.2013.08.016
dc.description.sourcetitleJournal of Chromatography A
dc.description.volume1308
dc.description.page166-171
dc.description.codenJCRAE
dc.identifier.isiut000324353100021
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