Please use this identifier to cite or link to this item:
|Title:||Rapid and sensitive profiling of tear wax ester species using high performance liquid chromatography coupled with tandem mass spectrometry||Authors:||Lam, S.M.
Multiple reaction monitoring (MRM)
|Issue Date:||20-Sep-2013||Citation:||Lam, S.M., Tong, L., Reux, B., Lear, M.J., Wenk, M.R., Shui, G. (2013-09-20). Rapid and sensitive profiling of tear wax ester species using high performance liquid chromatography coupled with tandem mass spectrometry. Journal of Chromatography A 1308 : 166-171. ScholarBank@NUS Repository. https://doi.org/10.1016/j.chroma.2013.08.016||Abstract:||A rapid and sensitive method was developed for quantitative profiling of wax esters (WEs) in human tear lipidome. Individual WE species was separated by liquid chromatography and detected by electrospray ionisation mass spectrometry using specific multiple reaction monitoring (MRM) scanning. Palmitoyl palmitate and in-house synthesized wax esters 13C18:1(oleic acid-1,2,3,7,8,9,10-13C7)C26:0 were used as internal standards for quantitation of WEs containing saturated and unsaturated fatty acids (FA), respectively. The limit of detection was approximately 70nmol/L. The linearity range of the liquid chromatography (LC)-MRM detection for WEs was about three orders of magnitude. Quantitative analyses of 141 individual WE in the human tear lipidome demonstrated that species comprising FA18:1 and FA16:1 each accounted for 47.7% and 24.0% (molar%) of total WE, while fatty alcohols in WEs of human tears ranged from 17 carbons to 32 carbons with predominant species represented by C24, C25 and C26. © 2013 Elsevier B.V.||Source Title:||Journal of Chromatography A||URI:||http://scholarbank.nus.edu.sg/handle/10635/110622||ISSN:||00219673||DOI:||10.1016/j.chroma.2013.08.016|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Sep 17, 2020
WEB OF SCIENCETM
checked on Sep 10, 2020
checked on Sep 19, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.