Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.nano.2013.05.009
DC Field | Value | |
---|---|---|
dc.title | Protective role of functionalized single walled carbon nanotubes enhance ex vivo expansion of hematopoietic stem and progenitor cells in human umbilical cord blood | |
dc.contributor.author | Bari, S. | |
dc.contributor.author | Chu, P.P.Y. | |
dc.contributor.author | Lim, A. | |
dc.contributor.author | Fan, X. | |
dc.contributor.author | Gay, F.P.H. | |
dc.contributor.author | Bunte, R.M. | |
dc.contributor.author | Lim, T.K.H. | |
dc.contributor.author | Li, S. | |
dc.contributor.author | Chiu, G.N.C. | |
dc.contributor.author | Hwang, W.Y.K. | |
dc.date.accessioned | 2014-11-26T09:05:04Z | |
dc.date.available | 2014-11-26T09:05:04Z | |
dc.date.issued | 2013-11 | |
dc.identifier.citation | Bari, S., Chu, P.P.Y., Lim, A., Fan, X., Gay, F.P.H., Bunte, R.M., Lim, T.K.H., Li, S., Chiu, G.N.C., Hwang, W.Y.K. (2013-11). Protective role of functionalized single walled carbon nanotubes enhance ex vivo expansion of hematopoietic stem and progenitor cells in human umbilical cord blood. Nanomedicine: Nanotechnology, Biology, and Medicine 9 (8) : 1304-1316. ScholarBank@NUS Repository. https://doi.org/10.1016/j.nano.2013.05.009 | |
dc.identifier.issn | 15499634 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/110614 | |
dc.description.abstract | In this study, carboxylic acid functionalized single walled carbon nanotubes (f-SWCNT-COOH) was shown to support the viability and ex vivo expansion of freeze-thawed, non-enriched hematopoietic stem and progenitor cells (HSPC) in human umbilical cord blood-mononucleated cells (UCB-MNC). Our in vitro experiments showed that f-SWCNT-COOH increased the viability of the CD45+ cells even without cytokine stimulation. It also reduced mitochondrial superoxides and caspase activity in CD45+ cells. f-SWCNT-COOH drastically reduced the proportions of CD45- cells in the non-enriched UCB-MNC. Phenotypic expression analysis and functional colony forming units (CFU) showed significant ex vivo expansion of HSPC, particularly of CD45+CD34+CD38- population and granulocyte-macrophage (GM) colonies, in f-SWCNT-COOH augmented cultures supplemented with basal cytokines. In vivo data suggested that f-SWCNT-COOH expanded UCB-MNC could repopulate immunodeficient mice models with minimal acute or sub-acute symptoms of graft-versus-host disease (GVHD) and f-SWCNT-COOH dependent toxicity. From the Clinical Editor: In this paper a novel method is presented by using single wall functionalized carbon nanotubes to enhance viability and ex vivo expansion of freeze-thawed, non-enriched hematopoietic stem and progenitor cells in human umbilical cord blood -mononucleated cells. Detailed data is presented about enhanced viability, including improved repopulation of immunodeficient mice models with minimal acute or sub-acute symptoms of graft-versus-host disease. © 2013 Elsevier Inc. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.nano.2013.05.009 | |
dc.source | Scopus | |
dc.subject | Ex Vivo Expansion of UCB | |
dc.subject | Functionalized Single Walled Carbon Nanotubes (f-SWNCT) | |
dc.subject | Hematopoietic Stem Cell Transplantation (HSCT) | |
dc.subject | Immunodeficient Mouse Model | |
dc.subject | Umbilical Cord Blood (UCB) | |
dc.type | Article | |
dc.contributor.department | DUKE-NUS GRADUATE MEDICAL SCHOOL S'PORE | |
dc.contributor.department | PHARMACY | |
dc.description.doi | 10.1016/j.nano.2013.05.009 | |
dc.description.sourcetitle | Nanomedicine: Nanotechnology, Biology, and Medicine | |
dc.description.volume | 9 | |
dc.description.issue | 8 | |
dc.description.page | 1304-1316 | |
dc.identifier.isiut | 000326118700018 | |
Appears in Collections: | Staff Publications |
Show simple item record
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.