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Title: G-CSF induces a potentially tolerant gene and immunophenotype profile in T cells in vivo
Authors: Toh, H.C.
Sun, L.
Soe, Y.
Wu, Y.
Phoon, Y.P.
Chia, W.K.
Wu, J.
Wong, K.Y.
Tan, P. 
Keywords: G-CSF
Immune tolerance
T cells
Issue Date: Jul-2009
Citation: Toh, H.C., Sun, L., Soe, Y., Wu, Y., Phoon, Y.P., Chia, W.K., Wu, J., Wong, K.Y., Tan, P. (2009-07). G-CSF induces a potentially tolerant gene and immunophenotype profile in T cells in vivo. Clinical Immunology 132 (1) : 83-92. ScholarBank@NUS Repository.
Abstract: G-CSF can induce functional immune tolerance in man. In this study, purified T cells from G-CSF-mobilized peripheral blood stem cell (PBSC) donors were analysed by gene expression profiling and immunophenotyping. Results suggested a predominantly immune tolerant profile with upregulation of genes related to Th2 and Treg cells, downregulation of genes associated with Th1 cells, cytotoxicity, antigen presentation and graft-versus-host disease (GVHD) and overexpression of negative regulators of Th17 differentiation. Immunophenotyping revealed that during G-CSF exposure donors had reduced levels of T cells with a Th17 phenotype (CD4+IL-17A+CCR6+IL-23R+), more than three times lower compared to normal controls. G-CSF also led to increased levels of CD4+CD25highCD45RO+ Treg cells. Furthermore, mRNA levels of RORγt, a Th17-specific transcription factor, decreased in T cells isolated from G-CSF-mobilized PBSC harvests. Th17 cells have been implicated in autoimmune diseases and GVHD pathophysiology. Our study is the first to report the effect of G-CSF on the Th17 subpopulation. © 2009 Elsevier Inc. All rights reserved.
Source Title: Clinical Immunology
ISSN: 15216616
DOI: 10.1016/j.clim.2009.03.509
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