Please use this identifier to cite or link to this item:
Title: Cotransplantation of Ex Vivo Expanded and Unexpanded Cord Blood Units in Immunodeficient Mice Using Insulin Growth Factor Binding Protein-2-Augmented Mesenchymal Cell Cocultures
Authors: Ong, L.M.
Fan, X.
Chu, P.P.Y.
Gay, F.P.H.
Bari, S.
Ang, J.M.L.
Li, Z.
Chen, J.
Lim, S.K.
Bunte, R.M. 
Hwang, W.Y.K.
Keywords: Double cord blood transplants
Ex vivo expansion
Graft versus host disease
Hematopoietic stem cells
Murine transplantation
Issue Date: May-2012
Citation: Ong, L.M., Fan, X., Chu, P.P.Y., Gay, F.P.H., Bari, S., Ang, J.M.L., Li, Z., Chen, J., Lim, S.K., Bunte, R.M., Hwang, W.Y.K. (2012-05). Cotransplantation of Ex Vivo Expanded and Unexpanded Cord Blood Units in Immunodeficient Mice Using Insulin Growth Factor Binding Protein-2-Augmented Mesenchymal Cell Cocultures. Biology of Blood and Marrow Transplantation 18 (5) : 674-682. ScholarBank@NUS Repository.
Abstract: Ex vivo expansion of cord blood (CB) hematopoietic stem cells and cotransplantation of 2 CB units (CBUs) could enhance the applicability of CB transplantation in adult patients. We report an immunodeficient mouse model for cotransplantation of ex vivo expanded and unexpanded human CB, showing enhanced CB engraftment and provide proof of concept for this transplantation strategy as a means of overcoming the limiting cell numbers in each CBU. CBUs were expanded in serum-free medium supplemented with stem cell factor, Flt-3 ligand, thrombopoietin, and insulin growth factor binding protein-2 together with mesenchymal stromal cell coculture. Unexpanded and expanded CB cells were cotransplanted by tail vein injection into 45 sublethally irradiated nonobese diabetic SCID-IL2γ-/- (NSG) mice. Submandibular bleeding was performed monthly, and mice were sacrificed 4 months after transplantation to analyze for human hematopoietic engraftment. Expansion of non-CD34+ selected CB cells yielded 40-fold expansion of CD34+ cells and 3.1-fold expansion of hematopoietic stem cells based on limiting dilution analysis of NSG engraftment. Mice receiving expanded grafts exhibited 4.30% human cell repopulation, compared with 0.92% in mice receiving only unexpanded grafts at equivalent starting cell doses, even though the unexpanded graft predominated in long-term hematopoiesis (P = .07). Ex vivo expanded grafts with lower initiating cell doses also showed equivalent engraftment to unexpanded grafts with higher cell dose (8.0% versus 7.9%; P = .93). In conclusion, ex vivo expansion resulted in enhanced CB engraftment despite eventual rejection by the unexpanded CBU. © 2012 American Society for Blood and Marrow Transplantation.
Source Title: Biology of Blood and Marrow Transplantation
ISSN: 10838791
DOI: 10.1016/j.bbmt.2012.01.001
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on May 7, 2021


checked on May 7, 2021

Page view(s)

checked on May 2, 2021

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.