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Title: A small-molecule dengue virus entry inhibitor
Authors: Wang, Q.-Y.
Patel, S.J.
Vangrevelinghe, E.
Hao, Y.X.
Rao, R.
Jaber, D.
Schul, W.
Gu, F.
Heudi, O.
Ngai, L.M.
Mee, K.P.
Wai, Y.P.
Keller, T.H.
Jacoby, E.
Vasudevan, S.G. 
Issue Date: May-2009
Citation: Wang, Q.-Y., Patel, S.J., Vangrevelinghe, E., Hao, Y.X., Rao, R., Jaber, D., Schul, W., Gu, F., Heudi, O., Ngai, L.M., Mee, K.P., Wai, Y.P., Keller, T.H., Jacoby, E., Vasudevan, S.G. (2009-05). A small-molecule dengue virus entry inhibitor. Antimicrobial Agents and Chemotherapy 53 (5) : 1823-1831. ScholarBank@NUS Repository.
Abstract: The incidence of dengue fever epidemics has increased dramatically over the last few decades. However, no vaccine or antiviral therapies are available. Therefore, the need for safe and effective antiviral drugs has become imperative. The entry of dengue virus into a host cell is mediated by its major envelope (E) protein. The crystal structure of the E protein reveals a hydrophobic pocket that is presumably important for low-pH-mediated membrane fusion. High-throughput docking with this hydrophobic pocket was performed, and hits were evaluated in cell-based assays. Compound 6 was identified as one of the inhibitors and had an average 50% effective concentration of 119 nM against dengue virus serotype 2 in a human cell line. Mechanism-of-action studies demonstrated that compound 6 acts at an early stage during dengue virus infection. It arrests dengue virus in vesicles that colocalize with endocytosed dextran and inhibits NS3 expression. The inhibitors described in this report can serve as molecular probes for the study of the entry of flavivirus into host cells. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
Source Title: Antimicrobial Agents and Chemotherapy
ISSN: 00664804
DOI: 10.1128/AAC.01148-08
Appears in Collections:Staff Publications

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