Please use this identifier to cite or link to this item:
|Title:||Viscoelastic behaviour of human mesenchymal stem cells||Authors:||Tan, S.C.W.
|Issue Date:||22-Jul-2008||Citation:||Tan, S.C.W., Pan, W.X., Ma, G., Cai, N., Leong, K.W., Liao, K. (2008-07-22). Viscoelastic behaviour of human mesenchymal stem cells. BMC Cell Biology 9 : -. ScholarBank@NUS Repository. https://doi.org/10.1186/1471-2121-9-40||Abstract:||Background: In this study, we have investigated the viscoelastic behaviour of individual human adult bone marrow-derived mesenchymal stem cells (hMSCs) and the role of F-actin filaments in maintaining these properties, using micropipette aspiration technique together with a standard linear viscoelastic solid model. Results: Under a room temperature of 20°C, the instantaneous and equilibrium Young's modulus, E0 and E∞, were found to be 886 ± 289 Pa and 372 ± 125 Pa, respectively, while the apparent viscosity, μ, was 2710 ± 1630 Pa·s. hMSCs treated with cytochalasin D up to 20 μM at 20°C registered significant drop of up to 84% in stiffness and increase of up to 255% in viscosity. At the physiological temperature of 37°C, E0 and E∞ have decreased by 42-66% whereas μ has increased by 95%, compared to the control. Majority of the hMSCs behave as viscoelastic solid with a rapid initial increase in aspiration length and it gradually levels out with time. Three other types of non-typical viscoelastic behavior of hMSCs were also seen. Conclusion: hMSCs behave as viscoelastic solid. Its viscoelstic behaviour are dependent on the structural integrity of the F-actin filaments and temperature. © 2008 Tan et al; licensee BioMed Central Ltd.||Source Title:||BMC Cell Biology||URI:||http://scholarbank.nus.edu.sg/handle/10635/110343||ISSN:||14712121||DOI:||10.1186/1471-2121-9-40|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on May 29, 2020
WEB OF SCIENCETM
checked on May 21, 2020
checked on May 30, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.