Please use this identifier to cite or link to this item: https://doi.org/10.1038/bjc.2014.45
Title: Tumour expression of leptin is associated with chemotherapy resistance and therapy-independent prognosis in gastro-oesophageal adenocarcinomas
Authors: Bain, G.H.
Collie-Duguid, E.
Murray, G.I.
Gilbert, F.J.
Denison, A.
McKiddie, F.
Ahearn, T.
Fleming, I.
Leeds, J.
Phull, P.
Park, K.
Nanthakumaran, S.
Grabsch, H.I.
Tan, P. 
Welch, A.
Schweiger, L.
Dahle-Smith, A.
Urquhart, G.
Finegan, M.
Petty, R.D.
Keywords: gastro-oesophageal adenocarcinoma
gene expression profiling
immunohistochemistry
leptin
predictive biomarker
prognostic biomarker
Issue Date: 18-Mar-2014
Citation: Bain, G.H., Collie-Duguid, E., Murray, G.I., Gilbert, F.J., Denison, A., McKiddie, F., Ahearn, T., Fleming, I., Leeds, J., Phull, P., Park, K., Nanthakumaran, S., Grabsch, H.I., Tan, P., Welch, A., Schweiger, L., Dahle-Smith, A., Urquhart, G., Finegan, M., Petty, R.D. (2014-03-18). Tumour expression of leptin is associated with chemotherapy resistance and therapy-independent prognosis in gastro-oesophageal adenocarcinomas. British Journal of Cancer 110 (6) : 1525-1534. ScholarBank@NUS Repository. https://doi.org/10.1038/bjc.2014.45
Abstract: Background: Cytotoxic chemotherapy remains the main systemic therapy for gastro-oesophageal adenocarcinoma, but resistance to chemotherapy is common, resulting in ineffective and often toxic treatment for patients. Predictive biomarkers for chemotherapy response would increase the probability of successful therapy, but none are currently recommended for clinical use. We used global gene expression profiling of tumour biopsies to identify novel predictive biomarkers for cytotoxic chemotherapy. Methods: Tumour biopsies from patients (n=14) with TNM stage IB-IV gastro-oesophageal adenocarcinomas receiving platinum-based combination chemotherapy were used as a discovery cohort and profiled with Affymetrix ST1.0 Exon Genechips. An independent cohort of patients (n=154) treated with surgery with or without neoadjuvant platinum combination chemotherapy and gastric adenocarcinoma cell lines (n=22) were used for qualification of gene expression profiling results by immunohistochemistry. A cisplatin-resistant gastric cancer cell line, AGS Cis5, and the oesophageal adenocarcinoma cell line, OE33, were used for in vitro validation investigations. Results: We identified 520 genes with differential expression (Mann-Whitney U, P
Source Title: British Journal of Cancer
URI: http://scholarbank.nus.edu.sg/handle/10635/110337
ISSN: 15321827
DOI: 10.1038/bjc.2014.45
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