Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0018326
Title: Transglutaminases (TGs) in ocular and periocular tissues: Effect of muscarinic agents on TGs in scleral fibroblasts
Authors: Barathi, V.A.
Weon, S.R.
Tan, Q.S.W.
Lin, K.J.
Tong, L. 
Beuerman, R.W.
Issue Date: 2011
Citation: Barathi, V.A., Weon, S.R., Tan, Q.S.W., Lin, K.J., Tong, L., Beuerman, R.W. (2011). Transglutaminases (TGs) in ocular and periocular tissues: Effect of muscarinic agents on TGs in scleral fibroblasts. PLoS ONE 6 (4) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0018326
Abstract: Objective: To investigate the expression of transglutaminases (TGs) in the ocular surface, the eyelid margin and associated glands and to determine effect of muscarinic agents on TGs in scleral fibroblasts (SF). Materials and Methods: Primary SFs cultured from mouse and human sclera were treated with atropine and carbachol for 5 days. Lysed cell RNA was used for real-time PCR, protein was used for Western blot analysis and TG-2 transamidase activity was measured by ELISA. Immunohistochemistry was done to determine the expression of TGases. Results: Immunohistochemistry and western blot confirmed the expression of TGs-1, 2, 3 and 5 proteins in cultured SFs and eye tissues. Real time PCR showed TG-1, 2, 5 transcript levels to be down regulated 3 fold (p
Source Title: PLoS ONE
URI: http://scholarbank.nus.edu.sg/handle/10635/110329
ISSN: 19326203
DOI: 10.1371/journal.pone.0018326
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