Please use this identifier to cite or link to this item: https://doi.org/10.1128/JVI.07081-11
Title: The magnitude of dengue virus NS1 protein secretion is strain dependent and does not correlate with severe pathologies in the mouse infection model
Authors: Watanabe, S.
Tan, K.H.
Rathore, A.P.S. 
Rozen-Gagnon, K.
Shuai, W.
Ruedl, C.
Vasudevan, S.G. 
Issue Date: May-2012
Citation: Watanabe, S., Tan, K.H., Rathore, A.P.S., Rozen-Gagnon, K., Shuai, W., Ruedl, C., Vasudevan, S.G. (2012-05). The magnitude of dengue virus NS1 protein secretion is strain dependent and does not correlate with severe pathologies in the mouse infection model. Journal of Virology 86 (10) : 5508-5514. ScholarBank@NUS Repository. https://doi.org/10.1128/JVI.07081-11
Abstract: There are conflicting data on the relationship between the level of secreted NS1 (sNS1), viremia, and disease severity upon dengue virus (DENV) infection in the clinical setting, and therefore, we examined this relationship in the widely accepted AG129 mouse model. Because of the failure of a routinely used NS1 detection kit to detect sNS1 of the mouse-adapted DENV2 strain, we screened 15 previously undescribed NS1 monoclonal antibodies and developed a robust capture enzyme-linked immunosorbent assay (ELISA) with detection sensitivity at the low nanogram level (0.2 ng/ml) using recombinant baculovirus-expressed sNS1 as well as sNS1 that was immunoaffinity purified from the various DENV2 strains employed in this study. Using this test, we demonstrated that increased viremia paralleled severe pathologies; however, sNS1 level did not correlate with viremia or severity. Furthermore, among the DENV2 strains that were tested, the level of NS1 secretion did not correspond to virus replication rate in vitro, at the cellular level. Together, our data indicate that the magnitude of NS1 secretion appears to be strain dependent and does not correlate with viral virulence in the AG129 mouse model. © 2012, American Society for Microbiology.
Source Title: Journal of Virology
URI: http://scholarbank.nus.edu.sg/handle/10635/110316
ISSN: 0022538X
DOI: 10.1128/JVI.07081-11
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

34
checked on Apr 5, 2020

WEB OF SCIENCETM
Citations

32
checked on Mar 25, 2020

Page view(s)

66
checked on Mar 28, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.