Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.ppat.1000178
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dc.titleThe core and accessory genomes of Burkholderia pseudomallei: Implications for human melioidosis
dc.contributor.authorSiew, H.S.
dc.contributor.authorYu, Y.
dc.contributor.authorChi, H.L.
dc.contributor.authorKaruturi, R.K.M.
dc.contributor.authorWuthiekanun, V.
dc.contributor.authorTuanyok, A.
dc.contributor.authorHui, H.C.
dc.contributor.authorOng, C.
dc.contributor.authorParamalingam, S.S.
dc.contributor.authorTan, G.
dc.contributor.authorTang, L.
dc.contributor.authorLau, G.
dc.contributor.authorEng, E.O.
dc.contributor.authorWoods, D.
dc.contributor.authorFeil, E.
dc.contributor.authorPeacock, S.J.
dc.contributor.authorTan, P.
dc.date.accessioned2014-11-26T08:30:50Z
dc.date.available2014-11-26T08:30:50Z
dc.date.issued2008-10
dc.identifier.citationSiew, H.S., Yu, Y., Chi, H.L., Karuturi, R.K.M., Wuthiekanun, V., Tuanyok, A., Hui, H.C., Ong, C., Paramalingam, S.S., Tan, G., Tang, L., Lau, G., Eng, E.O., Woods, D., Feil, E., Peacock, S.J., Tan, P. (2008-10). The core and accessory genomes of Burkholderia pseudomallei: Implications for human melioidosis. PLoS Pathogens 4 (10) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.ppat.1000178
dc.identifier.issn15537366
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/110301
dc.description.abstractNatural isolates of Burkholderia pseudomallei (Bp), the causative agent of melioidosis, can exhibit significant ecological flexibility that is likely reflective of a dynamic genome. Using whole-genome Bp microarrays, we examined patterns of gene presence and absence across 94 South East Asian strains isolated from a variety of clinical, environmental, or animal sources. 86% of the Bp K96243 reference genome was common to all the strains representing the Bp "core genome", comprising genes largely involved in essential functions (eg amino acid metabolism, protein translation). In contrast, 14% of the K96243 genome was variably present across the isolates. This Bp accessory genome encompassed multiple genomic islands (GIs), paralogous genes, and insertions/deletions, including three distinct lipopolysaccharide (LPS)-related gene clusters. Strikingly, strains recovered from cases of human melioidosis clustered on a tree based on accessory gene content, and were significantly more likely to harbor certain GIs compared to animal and environmental isolates. Consistent with the inference that the GIs may contribute to pathogenesis, experimental mutation of BPSS2053, a GI gene, reduced microbial adherence to human epithelial cells. Our results suggest that the Bp accessory genome is likely to play an important role in microbial adaptation and virulence. © 2008 Sim et al.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1371/journal.ppat.1000178
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentDUKE-NUS GRADUATE MEDICAL SCHOOL S'PORE
dc.description.doi10.1371/journal.ppat.1000178
dc.description.sourcetitlePLoS Pathogens
dc.description.volume4
dc.description.issue10
dc.description.page-
dc.identifier.isiut000261481100011
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