Please use this identifier to cite or link to this item: https://doi.org/10.1167/iovs.12-11466
Title: Optimization of subconjunctival biodegradable microfilms for sustained drug delivery to the anterior segment in a small animal model
Authors: Liu, Y.-C.
Peng, Y.
Lwin, N.C.
Wong, T.T.
Venkatraman, S.S.
Mehta, J.S. 
Keywords: Anterior segment
Drug delivery system
L-lactide-co-e-caprolactone] (PLC)
Poly[d
Issue Date: 2013
Citation: Liu, Y.-C., Peng, Y., Lwin, N.C., Wong, T.T., Venkatraman, S.S., Mehta, J.S. (2013). Optimization of subconjunctival biodegradable microfilms for sustained drug delivery to the anterior segment in a small animal model. Investigative Ophthalmology and Visual Science 54 (4) : 2607-2615. ScholarBank@NUS Repository. https://doi.org/10.1167/iovs.12-11466
Abstract: PURPOSE. We evaluated a biodegradable, sustained-release, prednisolone acetate (PA)-loaded poly[d,l-lactide-co-e-caprolactone] (PLC) drug delivery system on its biocompatibility, feasibility and release characteristics in vitro and in vivo. METHODS. Blank and 40% PA-loaded PLC microfilms with a diameter of 2 mm were fabricated, and the degradation and drug release profiles of the microfilms were characterized in vitro and in vivo. The microfilms were implanted into the subconjunctival space of Lewis rats (n 1/4 48). All eyes were assessed clinically using slit-lamp biomicroscopy, and graded with Hackett- McDonald ocular scoring system and anterior segment optical coherence tomography. Histologic and immunohistochemical analyses were performed comparing blank and PAloaded microfilm groups. PA concentrations in the aqueous humor were determined by HPLC. RESULTS. Subconjunctivally-implanted PLC microfilms were able to deliver PA in a sustained manner over 3 months, with a steady rate of 0.002 mg/d in vivo. Eyes with either blank or PAloaded implanted microfilms showed a very minimal inflammatory response at the insertion sites and mild degree of collagen encapsulation around the microfilms, with significantly less CD11c cells at 2 and 4 weeks (P 1/4 0.001 and P 1/4 0.002), and collagen formation at 2 weeks (P 1/4 0.001) in the PA-loaded microfilm group. Anterior chamber PA levels were achieved, with concentrations at 76.7 6 5.9, 70.3 6 2.3, and 42.7 6 4.1 ng/mL at 2, 4, and 12 weeks, respectively. CONCLUSIONS. PA-loaded PLC microfilms display good biocompatibility, feasibility, and desirable sustained drug release profiles, and have the potential to exhibit antifibrotic and antiinflammatory effects. This device is applicable to use in small animal models of anterior segment inflammation.
Source Title: Investigative Ophthalmology and Visual Science
URI: http://scholarbank.nus.edu.sg/handle/10635/110201
ISSN: 01460404
DOI: 10.1167/iovs.12-11466
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

13
checked on Oct 13, 2021

WEB OF SCIENCETM
Citations

13
checked on Oct 13, 2021

Page view(s)

92
checked on Oct 14, 2021

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.