Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.virusres.2013.11.010
DC Field | Value | |
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dc.title | Identification and molecular characterization of human antibody fragments specific for dengue NS5 protein | |
dc.contributor.author | Zhao, Y. | |
dc.contributor.author | Moreland, N.J. | |
dc.contributor.author | Tay, M.Y.F. | |
dc.contributor.author | Lee, C.C. | |
dc.contributor.author | Swaminathan, K. | |
dc.contributor.author | Vasudevan, S.G. | |
dc.date.accessioned | 2014-11-26T08:28:46Z | |
dc.date.available | 2014-11-26T08:28:46Z | |
dc.date.issued | 2014-01-22 | |
dc.identifier.citation | Zhao, Y., Moreland, N.J., Tay, M.Y.F., Lee, C.C., Swaminathan, K., Vasudevan, S.G. (2014-01-22). Identification and molecular characterization of human antibody fragments specific for dengue NS5 protein. Virus Research 179 (1) : 225-230. ScholarBank@NUS Repository. https://doi.org/10.1016/j.virusres.2013.11.010 | |
dc.identifier.issn | 01681702 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/110119 | |
dc.description.abstract | The multifunctional dengue nonstructural (NS) protein 5 from the four serotypes of dengue virus (DENV1-4) is essential for viral replication and harbors a methyl transferase (MTase) and a RNA-dependent RNA-polymerase domain (RdRp). There are limited comparative studies of NS5 from the four DENV serotypes and this is further hampered by a lack of cross-reactive NS5 antibodies. In this study, recombinant NS5 proteins were expressed, purified, enzymatically characterized, and used strategically as bait in biopanning experiments with a naïve human Fab phage-display library to identify serotype specific or cross-reactive Fab fragments. Using a combination of peptide competition ELISA and peptide phage display the epitopes of the cross-reactive Fabs were mapped to the first alpha helix of the MTase domain (5M1) and the priming loop of the RdRp domain (5R3). The epitope of a third, serotype-specific Fab (5M3) was mapped to aa19-30 of the DENV3 MTase domain. Together the recombinant proteins and specific antibodies will facilitate further mechanistic studies of the DENV replication complex. © 2013 Elsevier B.V. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.virusres.2013.11.010 | |
dc.source | Scopus | |
dc.subject | Antibody | |
dc.subject | Cross-reactivity | |
dc.subject | Dengue | |
dc.subject | NS5 | |
dc.subject | Phage display | |
dc.type | Article | |
dc.contributor.department | DUKE-NUS GRADUATE MEDICAL SCHOOL S'PORE | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.description.doi | 10.1016/j.virusres.2013.11.010 | |
dc.description.sourcetitle | Virus Research | |
dc.description.volume | 179 | |
dc.description.issue | 1 | |
dc.description.page | 225-230 | |
dc.description.coden | VIRED | |
dc.identifier.isiut | 000331677400026 | |
Appears in Collections: | Staff Publications |
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