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dc.titleIsolation of a novel chiral phytoestrogen breviflavone B from epimedium herb by a new approach of liquid chromatography
dc.contributor.authorSoh, S.F.
dc.contributor.authorTay, J.Y.
dc.contributor.authorLi, J.
dc.contributor.authorQian, L.
dc.contributor.authorYin, X.
dc.contributor.authorWei, Q.
dc.contributor.authorYang, H.
dc.contributor.authorWang, L.
dc.contributor.authorWang, D.
dc.contributor.authorYong, E.L.
dc.contributor.authorGong, Y.
dc.identifier.citationSoh, S.F., Tay, J.Y., Li, J., Qian, L., Yin, X., Wei, Q., Yang, H., Wang, L., Wang, D., Yong, E.L., Gong, Y. (2013). Isolation of a novel chiral phytoestrogen breviflavone B from epimedium herb by a new approach of liquid chromatography. Advanced Materials Research 663 : 303-307. ScholarBank@NUS Repository.
dc.description.abstractBreviflavone B is a novel chiral phytoestrogen that mimics the biological functions of 17β-estradiol (E2), an estrogen which is naturally produced in our body. It has exhibited a much higher estrogen receptor (ER) bioactivity than E2 or other phytoestrogens. Breviflavone B specifically activates ER at a low dose without activating other steroid receptors, and inhibits estrogen-stimulated breast cancer cell proliferation at high doses, making it a potential treatment option for breast cancer patients. In this report, a new liquid chromatography aprroach has been developed to isolate Breviflavone B from Epimedium Brevicornum herbal extracts. After passing through DIAION HP20 resin, the ethanolic extract of Epimedium Brevicornum was firstly fractionalized by a Diol column, then further separated by a C18 column using preparative HPLC, and finally purified by an ODS-MCR-HPS column in HPLC under gradient mode. In each stage of separation, the ER bioactivities of the each fraction were measured by estrogen receptor-driven reporter gene bioassays to guide the chromatographic fractionations. The results showed that the new combination of Diol and C18 and ODS-MCR-HPS column chromatography provided better separation selectivity than our previously-reported C18 column chromatography for isolation of Breviflavon B from Epemidium herbs. Pure Breviflavon B was successfully isolated from the ethanolic extract of Epimedium Brevicornum herb via this new approach of chromatographic fractionations guided by the bioassays. © (2013) Trans Tech Publications, Switzerland.
dc.subjectLiquid chromatography
dc.subjectNature product separation
dc.typeConference Paper
dc.contributor.departmentOBSTETRICS & GYNAECOLOGY
dc.description.sourcetitleAdvanced Materials Research
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