Please use this identifier to cite or link to this item: https://doi.org/10.4028/www.scientific.net/AMR.663.303
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dc.titleIsolation of a novel chiral phytoestrogen breviflavone B from epimedium herb by a new approach of liquid chromatography
dc.contributor.authorSoh, S.F.
dc.contributor.authorTay, J.Y.
dc.contributor.authorLi, J.
dc.contributor.authorQian, L.
dc.contributor.authorYin, X.
dc.contributor.authorWei, Q.
dc.contributor.authorYang, H.
dc.contributor.authorWang, L.
dc.contributor.authorWang, D.
dc.contributor.authorYong, E.L.
dc.contributor.authorGong, Y.
dc.date.accessioned2014-11-26T07:49:38Z
dc.date.available2014-11-26T07:49:38Z
dc.date.issued2013
dc.identifier.citationSoh, S.F., Tay, J.Y., Li, J., Qian, L., Yin, X., Wei, Q., Yang, H., Wang, L., Wang, D., Yong, E.L., Gong, Y. (2013). Isolation of a novel chiral phytoestrogen breviflavone B from epimedium herb by a new approach of liquid chromatography. Advanced Materials Research 663 : 303-307. ScholarBank@NUS Repository. https://doi.org/10.4028/www.scientific.net/AMR.663.303
dc.identifier.isbn9783037856000
dc.identifier.issn10226680
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/109756
dc.description.abstractBreviflavone B is a novel chiral phytoestrogen that mimics the biological functions of 17β-estradiol (E2), an estrogen which is naturally produced in our body. It has exhibited a much higher estrogen receptor (ER) bioactivity than E2 or other phytoestrogens. Breviflavone B specifically activates ER at a low dose without activating other steroid receptors, and inhibits estrogen-stimulated breast cancer cell proliferation at high doses, making it a potential treatment option for breast cancer patients. In this report, a new liquid chromatography aprroach has been developed to isolate Breviflavone B from Epimedium Brevicornum herbal extracts. After passing through DIAION HP20 resin, the ethanolic extract of Epimedium Brevicornum was firstly fractionalized by a Diol column, then further separated by a C18 column using preparative HPLC, and finally purified by an ODS-MCR-HPS column in HPLC under gradient mode. In each stage of separation, the ER bioactivities of the each fraction were measured by estrogen receptor-driven reporter gene bioassays to guide the chromatographic fractionations. The results showed that the new combination of Diol and C18 and ODS-MCR-HPS column chromatography provided better separation selectivity than our previously-reported C18 column chromatography for isolation of Breviflavon B from Epemidium herbs. Pure Breviflavon B was successfully isolated from the ethanolic extract of Epimedium Brevicornum herb via this new approach of chromatographic fractionations guided by the bioassays. © (2013) Trans Tech Publications, Switzerland.
dc.sourceScopus
dc.subjectLiquid chromatography
dc.subjectNature product separation
dc.subjectODS-MCR-HPS
dc.subjectTCM
dc.typeConference Paper
dc.contributor.departmentOBSTETRICS & GYNAECOLOGY
dc.description.doi10.4028/www.scientific.net/AMR.663.303
dc.description.sourcetitleAdvanced Materials Research
dc.description.volume663
dc.description.page303-307
dc.identifier.isiutNOT_IN_WOS
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