Please use this identifier to cite or link to this item: https://doi.org/10.1021/pr400650c
Title: Unlocking the puzzling biology of the Black Périgord truffle Tuber melanosporum
Authors: Islam, M.T.
Mohamedali, A.
Garg, G.
Khan, J.M.
Gorse, A.-D.
Parsons, J.
Marshall, P.
Ranganathan, S. 
Baker, M.S.
Keywords: black truffle
functional annotation
fungal proteomics
organoleptic
proteome
Issue Date: 6-Dec-2013
Citation: Islam, M.T., Mohamedali, A., Garg, G., Khan, J.M., Gorse, A.-D., Parsons, J., Marshall, P., Ranganathan, S., Baker, M.S. (2013-12-06). Unlocking the puzzling biology of the Black Périgord truffle Tuber melanosporum. Journal of Proteome Research 12 (12) : 5349-5356. ScholarBank@NUS Repository. https://doi.org/10.1021/pr400650c
Abstract: The black Périgord truffle (Tuber melanosporum Vittad.) is a highly prized food today, with its unique scent (i.e., perfume) and texture. Despite these attributes, it remains relatively poorly studied, lacking "omics" information to characterize its biology and biochemistry, especially changes associated with freshness and the proteins/metabolites responsible for its organoleptic properties. In this study, we have functionally annotated the truffle proteome from the 2010 T. melanosporum genome comprising 12 771 putative nonredundant proteins. Using sequential BLAST search strategies, we identified homologues for 2587 proteins with 2486 (96.0%) fungal homologues (available from http://biolinfo.org/protannotator/blacktruffle.php). A combined 1D PAGE and high-accuracy LC-MS/MS proteomic study was employed to validate the results of the functional annotation and identified 836 (6.5%) proteins, of which 47.5% (i.e., 397) were present in our bioinformatics studies. Our study, functionally annotating 6487 black Périgord truffle proteins and confirming 836 by proteomic experiments, is by far the most comprehensive study to date contributing significantly to the scientific community. This study has resulted in the functional characterization of novel proteins to increase our biological understanding of this organism and to uncover potential biomarkers of authenticity, freshness, and perfume maturation. © 2013 American Chemical Society.
Source Title: Journal of Proteome Research
URI: http://scholarbank.nus.edu.sg/handle/10635/109724
ISSN: 15353893
DOI: 10.1021/pr400650c
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

19
checked on Sep 24, 2022

WEB OF SCIENCETM
Citations

17
checked on Sep 15, 2022

Page view(s)

125
checked on Sep 22, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.