Please use this identifier to cite or link to this item: https://doi.org/10.1007/s00424-013-1347-4
Title: TRPM4 inhibition promotes angiogenesis after ischemic stroke
Authors: Loh, K.P.
Ng, G.
Yu, C.Y.
Fhu, C.K.
Yu, D.
Vennekens, R.
Nilius, B.
Soong, T.W. 
Liao, P.
Keywords: Angiogenesis
Capillary integrity
Ischemic stroke
TRPM4 channel
Issue Date: Mar-2014
Citation: Loh, K.P., Ng, G., Yu, C.Y., Fhu, C.K., Yu, D., Vennekens, R., Nilius, B., Soong, T.W., Liao, P. (2014-03). TRPM4 inhibition promotes angiogenesis after ischemic stroke. Pflugers Archiv European Journal of Physiology 466 (3) : 563-576. ScholarBank@NUS Repository. https://doi.org/10.1007/s00424-013-1347-4
Abstract: Transient receptor potential melastatin 4 (TRPM4) is a voltage-dependent, nonselective cation channel. Under pathological conditions, sustained activation of TRPM4 leads to oncotic cell death. Here, we report the upregulation of TRPM4 in vascular endothelium following hypoxia/ischemia in vitro and in vivo. In human umbilical vein endothelial cells, TRPM4 expression was increased at both the mRNA and protein levels following oxygen-glucose deprivation. Blocking TRPM4 with 9-phenanthrol greatly enhanced tube formation on Matrigel. In a rat permanent middle cerebral artery occlusion model, TRPM4 was upregulated in the vascular endothelium within the penumbra region after stroke. TRPM4 expression peaked 1 day post-occlusion and gradually decreased. In vivo siRNA-mediated TRPM4 silencing enhanced angiogenesis and improved capillary integrity. A twofold reduction in infarct volume and a substantial recovery of motor function were observed in animals receiving the siRNA treatment. Interestingly, the protective effect of TRPM4 suppression disappeared 5 days after stroke induction, indicating that TRPM4 upregulation is critical for cerebral damage during the acute phase of stroke. TRPM4 could be a potential therapeutic target for ischemic stroke. © 2013 Springer-Verlag Berlin Heidelberg.
Source Title: Pflugers Archiv European Journal of Physiology
URI: http://scholarbank.nus.edu.sg/handle/10635/109719
ISSN: 00316768
DOI: 10.1007/s00424-013-1347-4
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

55
checked on Dec 5, 2022

WEB OF SCIENCETM
Citations

50
checked on Dec 5, 2022

Page view(s)

162
checked on Nov 24, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.