Please use this identifier to cite or link to this item: https://doi.org/10.1074/jbc.M110.162438
Title: Syntaxin 16 binds to cystic fibrosis transmembrane conductance regulator and regulates its membrane trafficking in epithelial cells
Authors: Gee, H.Y.
Tang, B.L. 
Kim, K.H.
Lee, M.G.
Issue Date: 12-Nov-2010
Citation: Gee, H.Y., Tang, B.L., Kim, K.H., Lee, M.G. (2010-11-12). Syntaxin 16 binds to cystic fibrosis transmembrane conductance regulator and regulates its membrane trafficking in epithelial cells. Journal of Biological Chemistry 285 (46) : 35519-35527. ScholarBank@NUS Repository. https://doi.org/10.1074/jbc.M110.162438
Abstract: The cystic fibrosis transmembrane conductance regulator (CFTR) is a key membrane protein in the complex network of epithelial ion transporters regulating epithelial permeability. Syntaxins are one of the major determinants in the intracellular trafficking and membrane targeting of secretory proteins. In the present study we demonstrate the biochemical and functional association between CFTR and syntaxin 16 (STX16) that mediates vesicle transport within the early/late endosomes and trans-Golgi network. Immunoprecipitation experiments in rat colon and T84 human colonic epithelial cells indicate that STX16 associates with CFTR. Further analyses using the domain-specific pulldown assay reveal that the helix domain of STX16 directly interacts with the N-terminal region of CFTR. Immunostainings in rat colon and T84 cells show that CFTR and STX16 highly co-localize at the apical and subapical regions of epithelial cells. Interestingly, CFTR-associated chloride current was reduced by the knockdown of STX16 expression in T84 cells. Surface biotinylation and recycling assays indicate that the reduction in CFTR chloride current is due to decreased CFTR expression on the plasma membrane. These results suggest that STX16 mediates recycling of CFTR and constitutes an important component ofCFTRtrafficking machinery in intestinal epithelial cells. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.
Source Title: Journal of Biological Chemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/109679
ISSN: 00219258
DOI: 10.1074/jbc.M110.162438
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