Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep04619
Title: Reduced growth and proliferation dynamics of nasal epithelial stem/progenitor cells in nasal polyps in vitro
Authors: Yu, X.M.
Li, C.W. 
Chao, S.S.
Li, Y.Y.
Yan, Y.
Zhao, X.N.
Yu, F.G.
Liu, J.
Shen, L.
Pan, X.L.
Shi, L.
Wang, D.Y.
Issue Date: 9-Apr-2014
Citation: Yu, X.M., Li, C.W., Chao, S.S., Li, Y.Y., Yan, Y., Zhao, X.N., Yu, F.G., Liu, J., Shen, L., Pan, X.L., Shi, L., Wang, D.Y. (2014-04-09). Reduced growth and proliferation dynamics of nasal epithelial stem/progenitor cells in nasal polyps in vitro. Scientific Reports 4 : -. ScholarBank@NUS Repository. https://doi.org/10.1038/srep04619
Abstract: Basal cells in nasal epithelium have stemness/progenitor characters and play essential roles in the epithelial remodeling in nasal polyps (NP). We investigate whether the human nasal epithelial stem/progenitor cells (hNESPCs) from patients with NP are inherently distinct from those obtained from healthy controls. Epithelial basal cells were isolated and cultured for four passages from NP tissues and control nasal mucosa. hNESPCs from controls were stained positively with stem cell marker p63 and KRT5 and presented a consistent high Ki67 expression level over four passages. In contrast, hNESPCs from NP patients showed: i). a reduced growth and proliferation rate at each passage by evaluating colony-forming efficiency and doubling time; ii). a lower percentage of Ki67 + cells among p63 + cells in the colonies in late passages, which was also confirmed by immunostaining in the NP tissues. Thus reduced growth/proliferation dynamics in hNESPCs from NP could be an important pathological phenomenon in NP development.
Source Title: Scientific Reports
URI: http://scholarbank.nus.edu.sg/handle/10635/109572
ISSN: 20452322
DOI: 10.1038/srep04619
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