Please use this identifier to cite or link to this item: https://doi.org/10.1074/jbc.M110.108241
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dc.titleParkin enhances the expression of cyclin-dependent kinase 6 and negatively regulates the proliferation of breast cancer cells
dc.contributor.authorTay, S.-P.
dc.contributor.authorYeo, C.W.S.
dc.contributor.authorChai, C.
dc.contributor.authorChua, P.-J.
dc.contributor.authorTan, H.-M.
dc.contributor.authorAng, A.X.Y.
dc.contributor.authorYip, D.L.H.
dc.contributor.authorSung, J.-X.
dc.contributor.authorTan, P.H.
dc.contributor.authorBay, B.-H.
dc.contributor.authorWong, S.-H.
dc.contributor.authorTang, C.
dc.contributor.authorTan, J.M.M.
dc.contributor.authorLim, K.-L.
dc.date.accessioned2014-11-26T07:46:46Z
dc.date.available2014-11-26T07:46:46Z
dc.date.issued2010-09-17
dc.identifier.citationTay, S.-P., Yeo, C.W.S., Chai, C., Chua, P.-J., Tan, H.-M., Ang, A.X.Y., Yip, D.L.H., Sung, J.-X., Tan, P.H., Bay, B.-H., Wong, S.-H., Tang, C., Tan, J.M.M., Lim, K.-L. (2010-09-17). Parkin enhances the expression of cyclin-dependent kinase 6 and negatively regulates the proliferation of breast cancer cells. Journal of Biological Chemistry 285 (38) : 29231-29238. ScholarBank@NUS Repository. https://doi.org/10.1074/jbc.M110.108241
dc.identifier.issn00219258
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/109507
dc.description.abstractAlthough mutations in the parkin gene are frequently associated with familial Parkinsonism, emerging evidence suggests that parkin also plays a role in cancers as a putative tumor suppressor. Supporting this, we show here that parkin expression is dramatically reduced in several breast cancer-derived cell lines as well as in primary breast cancer tissues. Importantly, we found that ectopic parkin expression in parkin-deficient breast cancer cells mitigates their proliferation rate both in vitro and in vivo, as well as reduces the capacity of these cells to migrate. Cell cycle analysis revealed the arrestment of a significant percentage of parkin-expressing breast cancer cells at the G1-phase. However, we did not observe significant changes in the levels of the G1-associated cyclin D1 and E. On the other hand, the level of cyclin-dependent kinase 6 (CDK6) is dramatically and selectively elevated in parkin-expressing breast cancer cells, the extent of which correlates well with the expression of parkin. Interestingly, a recent study demonstrated that CDK6 restrains the proliferation of breast cancer cells. Taken together, our results support a negative role for parkin in tumorigenesis and provide a potential mechanism by which parkin exerts its suppressing effects on breast cancer cell proliferation. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1074/jbc.M110.108241
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentDUKE-NUS GRADUATE MEDICAL SCHOOL S'PORE
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1074/jbc.M110.108241
dc.description.sourcetitleJournal of Biological Chemistry
dc.description.volume285
dc.description.issue38
dc.description.page29231-29238
dc.description.codenJBCHA
dc.identifier.isiut000281740100024
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