Please use this identifier to cite or link to this item:
Title: Parkin enhances the expression of cyclin-dependent kinase 6 and negatively regulates the proliferation of breast cancer cells
Authors: Tay, S.-P.
Yeo, C.W.S.
Chai, C. 
Chua, P.-J.
Tan, H.-M.
Ang, A.X.Y.
Yip, D.L.H.
Sung, J.-X.
Tan, P.H.
Bay, B.-H.
Wong, S.-H. 
Tang, C.
Tan, J.M.M.
Lim, K.-L. 
Issue Date: 17-Sep-2010
Citation: Tay, S.-P., Yeo, C.W.S., Chai, C., Chua, P.-J., Tan, H.-M., Ang, A.X.Y., Yip, D.L.H., Sung, J.-X., Tan, P.H., Bay, B.-H., Wong, S.-H., Tang, C., Tan, J.M.M., Lim, K.-L. (2010-09-17). Parkin enhances the expression of cyclin-dependent kinase 6 and negatively regulates the proliferation of breast cancer cells. Journal of Biological Chemistry 285 (38) : 29231-29238. ScholarBank@NUS Repository.
Abstract: Although mutations in the parkin gene are frequently associated with familial Parkinsonism, emerging evidence suggests that parkin also plays a role in cancers as a putative tumor suppressor. Supporting this, we show here that parkin expression is dramatically reduced in several breast cancer-derived cell lines as well as in primary breast cancer tissues. Importantly, we found that ectopic parkin expression in parkin-deficient breast cancer cells mitigates their proliferation rate both in vitro and in vivo, as well as reduces the capacity of these cells to migrate. Cell cycle analysis revealed the arrestment of a significant percentage of parkin-expressing breast cancer cells at the G1-phase. However, we did not observe significant changes in the levels of the G1-associated cyclin D1 and E. On the other hand, the level of cyclin-dependent kinase 6 (CDK6) is dramatically and selectively elevated in parkin-expressing breast cancer cells, the extent of which correlates well with the expression of parkin. Interestingly, a recent study demonstrated that CDK6 restrains the proliferation of breast cancer cells. Taken together, our results support a negative role for parkin in tumorigenesis and provide a potential mechanism by which parkin exerts its suppressing effects on breast cancer cell proliferation. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.
Source Title: Journal of Biological Chemistry
ISSN: 00219258
DOI: 10.1074/jbc.M110.108241
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
jbc.M110.108241.pdf1.96 MBAdobe PDF




checked on Jan 30, 2023


checked on Jan 23, 2023

Page view(s)

checked on Jan 26, 2023


checked on Jan 26, 2023

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.