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Title: MRT letter: Micro- to nanoscale sample collection for high throughput microscopy
Authors: Cheong, B.H.-P.
Liew, O.W. 
Wah Ng, T.
Keywords: Coverslips
High throughput microscopy
Object collection
Squeeze flow
Issue Date: Aug-2013
Citation: Cheong, B.H.-P., Liew, O.W., Wah Ng, T. (2013-08). MRT letter: Micro- to nanoscale sample collection for high throughput microscopy. Microscopy Research and Technique 76 (8) : 767-773. ScholarBank@NUS Repository.
Abstract: In high throughput microscopy, it is often assumed that the objects under investigation are fixed spatially. In addition, it is also presumed that the objects are sufficiently populated, otherwise there will be need to search through vast tracks of field of views before any recording can be done. The ability to collect objects at one location in the hydrated state is thus desirable and this is a challenge when the density of target objects in a sample is very low. In this work, we report that the generation of a squeezing flow from a circular coverslip compressing on suspensions is able to collect particulate (microbeads, fluorescent nanobeads and live algal cells) and non-particulate (EGFP) objects at the rim region of the coverslip. With a coverslip of 13 mm diameter, volumes between 2 μL and 4 μL were found to completely fill the coverslip without breaching the rims. Sample compression speeds between 100 μm/s and 1000 μm/s did not have any effect on object collection outcomes. In effect, the simple placement of coverslips on top the drop of sample by hand without a motorized translator was found to produce similar collection outcomes. Quantitative measurements confirmed that all the objects investigated were displaced and relocated at the rim regions to a very high degree. © 2013 Wiley Periodicals, Inc.
Source Title: Microscopy Research and Technique
ISSN: 1059910X
DOI: 10.1002/jemt.22238
Appears in Collections:Staff Publications

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