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https://doi.org/10.1371/journal.pone.0041523
Title: | MicroRNA-7 inhibits epithelial-to-mesenchymal transition and metastasis of breast cancer cells via targeting FAK expression | Authors: | Kong, X. Li, G. Yuan, Y. He, Y. Wu, X. Zhang, W. Wu, Z. Chen, T. Wu, W. Lobie, P.E. Zhu, T. |
Issue Date: | 2-Aug-2012 | Citation: | Kong, X., Li, G., Yuan, Y., He, Y., Wu, X., Zhang, W., Wu, Z., Chen, T., Wu, W., Lobie, P.E., Zhu, T. (2012-08-02). MicroRNA-7 inhibits epithelial-to-mesenchymal transition and metastasis of breast cancer cells via targeting FAK expression. PLoS ONE 7 (8) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0041523 | Abstract: | Focal adhesion kinase (FAK) is an important mediator of extracellular matrix integrin signaling, cell motility, cell proliferation and cell survival. Increased FAK expression is observed in a variety of solid human tumors and increased FAK expression and activity frequently correlate with metastatic disease and poor prognosis. Herein we identify miR-7 as a direct regulator of FAK expression. miR-7 expression is decreased in malignant versus normal breast tissue and its expression correlates inversely with metastasis in human breast cancer patients. Forced expression of miR-7 produced increased E-CADHERIN and decreased FIBRONECTIN and VIMENTIN expression in breast cancer cells. The levels of miR-7 expression was positively correlated with E-CADHERIN mRNA and negatively correlated with VIMENTIN mRNA levels in breast cancer samples. Forced expression of miR-7 in aggressive breast cancer cell lines suppressed tumor cell monolayer proliferation, anchorage independent growth, three-dimensional growth in Matrigel, migration and invasion. Conversely, inhibition of miR-7 in the HBL-100 mammary epithelial cell line promoted cell proliferation and anchorage independent growth. Rescue of FAK expression reversed miR-7 suppression of migration and invasion. miR-7 also inhibited primary breast tumor development, local invasion and metastatic colonization of breast cancer xenografts. Thus, miR-7 expression is decreased in metastatic breast cancer, correlates with the level of epithelial differentiation of the tumor and inhibits metastatic progression. © 2012 Kong et al. | Source Title: | PLoS ONE | URI: | http://scholarbank.nus.edu.sg/handle/10635/109464 | ISSN: | 19326203 | DOI: | 10.1371/journal.pone.0041523 |
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