Please use this identifier to cite or link to this item: https://doi.org/10.1007/978-1-61779-012-6_13
Title: iTRAQ™ labeling coupled with LC-MALDI mass spectrometry for monitoring temporal response of colorectal cancer cells to butyrate treatment.
Authors: Tan, H.T. 
Lim, T.K.
Chung, M.C.
Lin, Q.
Issue Date: 2011
Citation: Tan, H.T.,Lim, T.K.,Chung, M.C.,Lin, Q. (2011). iTRAQ™ labeling coupled with LC-MALDI mass spectrometry for monitoring temporal response of colorectal cancer cells to butyrate treatment.. Methods in molecular biology (Clifton, N.J.) 716 : 207-224. ScholarBank@NUS Repository. https://doi.org/10.1007/978-1-61779-012-6_13
Abstract: Mass spectrometry (MS)-based quantitative proteomics plays important roles in drug discovery. In this chapter, we describe a stable isotope labeling technique which employs 4-plex iTRAQ(™) isobaric reagents coupled with two-dimensional (2-D) liquid chromatography (LC) and MALDI-TOF/TOF MS, for a temporal study of HCT-116 colon carcinoma cells treated with butyrate. Butyrate is a short-chain fatty acid fermentation by-product of fiber that can induce temporal cell maturation, from the early phase of growth arrest, to differentiation, and to the activation of apoptotic cascades. Our quantitative proteomics study uncovered several integrated cellular processes and pathways involved in growth arrest, apoptosis, and metastasis. Selected protein targets are validated by real-time PCR and western blotting.
Source Title: Methods in molecular biology (Clifton, N.J.)
URI: http://scholarbank.nus.edu.sg/handle/10635/109428
ISSN: 19406029
DOI: 10.1007/978-1-61779-012-6_13
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