Please use this identifier to cite or link to this item: https://doi.org/10.1186/2049-1891-4-28
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dc.titleHypoxia promotes cell proliferation by modulating E2F1 in chicken pulmonary arterial smooth muscle cells
dc.contributor.authorYang, Y.
dc.contributor.authorSun, F.
dc.contributor.authorZhang, C.
dc.contributor.authorWang, H.
dc.contributor.authorWu, G.
dc.contributor.authorWu, Z.
dc.date.accessioned2014-11-26T07:45:13Z
dc.date.available2014-11-26T07:45:13Z
dc.date.issued2013-07-31
dc.identifier.citationYang, Y., Sun, F., Zhang, C., Wang, H., Wu, G., Wu, Z. (2013-07-31). Hypoxia promotes cell proliferation by modulating E2F1 in chicken pulmonary arterial smooth muscle cells. Journal of Animal Science and Biotechnology 4 (1) : -. ScholarBank@NUS Repository. https://doi.org/10.1186/2049-1891-4-28
dc.identifier.issn16749782
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/109385
dc.description.abstractIn this study, we sought to investigate the expression of the transcription factor E2F1 in chicken pulmonary arterial smooth muscle cells upon hypoxia exposure, as well as the role that E2F1 played in the regulation of cell proliferation. Isolated chicken pulmonary arterial smooth muscle cells were subjected to hypoxia or normoxia for indicated time points. Cell viability, DNA synthesis, cell cycle profile, and expression of E2F1 were analyzed. The results showed that hypoxia promoted cell proliferation and DNA synthesis which was accompanied by an increased S phase entry and upregulation of E2F1 at mRNA and protein levels. Using siRNA technology, we demonstrated that gene inactivation of endogenous E2F1 abolished hypoxia-induced cell proliferation, DNA synthesis, and S phase entry compared with negative siRNA transfected cells. These results suggest that hypoxia-induced proliferation is mediated by inducing E2F1 in chicken pulmonary arterial smooth muscle cells. © 2013 Yang et al.; licensee BioMed Central Ltd.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1186/2049-1891-4-28
dc.sourceScopus
dc.subjectE2F1
dc.subjectHypoxia
dc.subjectProliferation
dc.subjectPulmonary arterial smooth muscle cells
dc.typeArticle
dc.contributor.departmentPHYSIOLOGY
dc.description.doi10.1186/2049-1891-4-28
dc.description.sourcetitleJournal of Animal Science and Biotechnology
dc.description.volume4
dc.description.issue1
dc.description.page-
dc.identifier.isiut000333181400001
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