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Title: Fugu genome does not contain mitochondrial pseudogenes
Authors: Venkatesh, B. 
Dandona, N.
Brenner, S.
Keywords: Fugu
Mitochondrial pseudogene
Teleost fish
Whole-genome shotgun sequencing
Issue Date: Feb-2006
Citation: Venkatesh, B., Dandona, N., Brenner, S. (2006-02). Fugu genome does not contain mitochondrial pseudogenes. Genomics 87 (2) : 307-310. ScholarBank@NUS Repository.
Abstract: Contrary to previous observations that fish genomes are devoid of nuclear mitochondrial pseudogenes, a genome-wide survey identified a large number of "recent" and "ancient" nuclear mitochondrial DNA fragments (Numts) in the whole-genome sequences of the fugu (Takifugu rubripes), Tetraodon nigroviridis, and zebrafish (Danio rerio). We have analyzed the latest assembly (v4.0) of the fugu genome and show that, like the Anopheles genome, the fugu nuclear genome does not contain mitochondrial pseudogenes. Fugu assembly v4.0 contains a single scaffold representing the near complete sequence of the fugu mitochondria. The "recent" Numts identified by the previous study in fugu assembly v2.0 are in fact shotgun sequences of mitochondrial DNA that were misassembled with the nuclear sequences, whereas the "ancient" Numts appear to be the result of spurious matches. It is likely that the Numts identified in the genomes of Tetraodon and zebrafish are also similar artifacts. Shotgun sequences of whole genomes often include some mitochondrial sequences. Therefore, any Numts identified in shotgun-sequence assemblies should be verified by Southern hybridization or PCR amplification. © 2005 Elsevier Inc. All rights reserved.
Source Title: Genomics
ISSN: 08887543
DOI: 10.1016/j.ygeno.2005.11.007
Appears in Collections:Staff Publications

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