Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.ejphar.2012.01.002
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dc.titleFisetin, a bioactive flavonol, attenuates allergic airway inflammation through negative regulation of NF-κB
dc.contributor.authorGoh, F.Y.
dc.contributor.authorUpton, N.
dc.contributor.authorGuan, S.
dc.contributor.authorCheng, C.
dc.contributor.authorShanmugam, M.K.
dc.contributor.authorSethi, G.
dc.contributor.authorLeung, B.P.
dc.contributor.authorWong, W.S.F.
dc.date.accessioned2014-11-26T07:44:44Z
dc.date.available2014-11-26T07:44:44Z
dc.date.issued2012-03-15
dc.identifier.citationGoh, F.Y., Upton, N., Guan, S., Cheng, C., Shanmugam, M.K., Sethi, G., Leung, B.P., Wong, W.S.F. (2012-03-15). Fisetin, a bioactive flavonol, attenuates allergic airway inflammation through negative regulation of NF-κB. European Journal of Pharmacology 679 (1-3) : 109-116. ScholarBank@NUS Repository. https://doi.org/10.1016/j.ejphar.2012.01.002
dc.identifier.issn00142999
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/109347
dc.description.abstractPersistent activation of nuclear factor-κB (NF-κB) has been associated with the development of asthma. Fisetin (3,7,3′,4′- tetrahydroxyflavone), a naturally occurring bioactive flavonol, has been shown to inhibit NF-κB activity. We hypothesized that fisetin may attenuate allergic asthma via negative regulation of the NF-κB activity. Female BALB/c mice sensitized and challenged with ovalbumin developed airway inflammation. Bronchoalveolar lavage fluid was assessed for total and differential cell counts, and cytokine and chemokine levels. Lung tissues were examined for cell infiltration and mucus hypersecretion, and the expression of inflammatory biomarkers. Airway hyperresponsiveness was monitored by direct airway resistance analysis. Fisetin dose-dependently inhibited ovalbumin-induced increases in total cell count, eosinophil count, and IL-4, IL-5 and IL-13 levels recovered in bronchoalveolar lavage fluid. It attenuated ovalbumin-induced lung tissue eosinophilia and airway mucus production, mRNA expression of adhesion molecules, chitinase, IL-17, IL-33, Muc5ac and inducible nitric oxide synthase in lung tissues, and airway hyperresponsiveness to methacholine. Fisetin blocked NF-κB subunit p65 nuclear translocation and DNA-binding activity in the nuclear extracts from lung tissues of ovalbumin-challenged mice. In normal human bronchial epithelial cells, fisetin repressed TNF-α-induced NF-κB-dependent reporter gene expression. Our findings implicate a potential therapeutic value of fisetin in the treatment of asthma through negative regulation of NF-κB pathway. © 2012 Elsevier B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.ejphar.2012.01.002
dc.sourceScopus
dc.subjectAirway hyperresponsiveness
dc.subjectFlavonol
dc.subjectLung epithelium
dc.subjectOvalbumin
dc.subjectTNF-α
dc.typeArticle
dc.contributor.departmentPHARMACOLOGY
dc.description.doi10.1016/j.ejphar.2012.01.002
dc.description.sourcetitleEuropean Journal of Pharmacology
dc.description.volume679
dc.description.issue1-3
dc.description.page109-116
dc.description.codenEJPHA
dc.identifier.isiut000300731900015
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