Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jpba.2013.08.020
Title: Determination of androgen receptor degradation enhancer ASC-J9® in mouse sera and organs with liquid chromatography tandem mass spectrometry
Authors: Soh, S.F.
Huang, C.-K.
Lee, S.O.
Xu, D.
Yeh, S.
Li, J.
Yong, E.L.
Gong, Y. 
Chang, C.
Keywords: Androgen receptor
ASC-J9®
Distribution of drug
Liquid chromatography tandem mass spectrometry
Pharmacokinetics
Issue Date: 2014
Citation: Soh, S.F., Huang, C.-K., Lee, S.O., Xu, D., Yeh, S., Li, J., Yong, E.L., Gong, Y., Chang, C. (2014). Determination of androgen receptor degradation enhancer ASC-J9® in mouse sera and organs with liquid chromatography tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis 88 : 117-112. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jpba.2013.08.020
Abstract: A novel androgen receptor (AR) degradation enhancer ASC-J9® has displayed beneficial effects during the in vitro and in vivo studies for treatment of prostate cancer, liver cancer, bladder cancer and spinal and bulbar muscular atrophy (SBMA). It works mainly via the degradation of AR with minimal side effects on the tested mice. Here we developed a fast, robust and more sensitive method for the quantification of ASC-J9® in 100μL of mouse serum by using liquid chromatography tandem mass spectrometry (LC-MS/MS). The limit of quantification (LOQ) was found to be 5nM for ASCJ9®. This method was successfully applied to investigate the pharmacokinetics of ASC-J9® in mice serum samples and also the distribution of the drug in various mice organs after single dose injection with results showing that ASC-J9® could be quickly absorbed in vivo and had a relatively slow elimination half-life of 5.45h. The ASC-J9® also exhibited a higher tendency to accumulate in organs such as liver, testes and prostate. © 2013 Elsevier B.V.
Source Title: Journal of Pharmaceutical and Biomedical Analysis
URI: http://scholarbank.nus.edu.sg/handle/10635/109292
ISSN: 07317085
DOI: 10.1016/j.jpba.2013.08.020
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

13
checked on Jul 19, 2019

WEB OF SCIENCETM
Citations

12
checked on Jul 19, 2019

Page view(s)

74
checked on Jul 19, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.