Please use this identifier to cite or link to this item: https://doi.org/10.1101/gr.113555.110
DC FieldValue
dc.titleComprehensive long-span paired-end-tag mapping reveals characteristic patterns of structural variations in epithelial cancer genomes
dc.contributor.authorHillmer, A.M.
dc.contributor.authorYao, F.
dc.contributor.authorInaki, K.
dc.contributor.authorLee, W.H.
dc.contributor.authorAriyaratne, P.N.
dc.contributor.authorTeo, A.S.M.
dc.contributor.authorWoo, X.Y.
dc.contributor.authorZhang, Z.
dc.contributor.authorZhao, H.
dc.contributor.authorUkil, L.
dc.contributor.authorChen, J.P.
dc.contributor.authorZhu, F.
dc.contributor.authorSo, J.B.Y.
dc.contributor.authorSalto-Tellez, M.
dc.contributor.authorPoh, W.T.
dc.contributor.authorZawack, K.F.B.
dc.contributor.authorNagarajan, N.
dc.contributor.authorGao, S.
dc.contributor.authorLi, G.
dc.contributor.authorKumar, V.
dc.contributor.authorLim, H.P.J.
dc.contributor.authorSia, Y.Y.
dc.contributor.authorChan, C.S.
dc.contributor.authorLeong, S.T.
dc.contributor.authorNeo, S.C.
dc.contributor.authorChoi, P.S.D.
dc.contributor.authorThoreau, H.
dc.contributor.authorTan, P.B.O.
dc.contributor.authorShahab, A.
dc.contributor.authorRuan, X.
dc.contributor.authorBergh, J.
dc.contributor.authorHall, P.
dc.contributor.authorCacheux-Rataboul, V.
dc.contributor.authorWei, C.-L.
dc.contributor.authorYeoh, K.G.
dc.contributor.authorSung, W.-K.
dc.contributor.authorBourque, G.
dc.contributor.authorLiu, E.T.
dc.contributor.authorRuan, Y.
dc.date.accessioned2014-11-26T07:43:48Z
dc.date.available2014-11-26T07:43:48Z
dc.date.issued2011-05
dc.identifier.citationHillmer, A.M., Yao, F., Inaki, K., Lee, W.H., Ariyaratne, P.N., Teo, A.S.M., Woo, X.Y., Zhang, Z., Zhao, H., Ukil, L., Chen, J.P., Zhu, F., So, J.B.Y., Salto-Tellez, M., Poh, W.T., Zawack, K.F.B., Nagarajan, N., Gao, S., Li, G., Kumar, V., Lim, H.P.J., Sia, Y.Y., Chan, C.S., Leong, S.T., Neo, S.C., Choi, P.S.D., Thoreau, H., Tan, P.B.O., Shahab, A., Ruan, X., Bergh, J., Hall, P., Cacheux-Rataboul, V., Wei, C.-L., Yeoh, K.G., Sung, W.-K., Bourque, G., Liu, E.T., Ruan, Y. (2011-05). Comprehensive long-span paired-end-tag mapping reveals characteristic patterns of structural variations in epithelial cancer genomes. Genome Research 21 (5) : 665-675. ScholarBank@NUS Repository. https://doi.org/10.1101/gr.113555.110
dc.identifier.issn10889051
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/109265
dc.description.abstractSomatic genome rearrangements are thought to play important roles in cancer development. We optimized a long-span paired-end-tag (PET) sequencing approach using 10-Kb genomic DNA inserts to study human genome structural variations (SVs). The use of a 10-Kb insert size allows the identification of breakpoints within repetitive or homology-containing regions of a few kilobases in size and results in a higher physical coverage compared with small insert libraries with the same sequencing effort. We have applied this approach to comprehensively characterize the SVs of 15 cancer and two noncancer genomes and used a filtering approach to strongly enrich for somatic SVs in the cancer genomes. Our analyses revealed that most inversions, deletions, and insertions are germ-line SVs, whereas tandem duplications, unpaired inversions, interchromosomal translocations, and complex rearrangements are over-represented among somatic rearrangements in cancer genomes. We demonstrate that the quantitative and connective nature of DNA-PET data is precise in delineating the genealogy of complex rearrangement events, we observe signatures that are compatible with breakage-fusion-bridge cycles, and we discover that large duplications are among the initial rearrangements that trigger genome instability for extensive amplification in epithelial cancers. © 2011 by Cold Spring Harbor Laboratory Press.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1101/gr.113555.110
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOCHEMISTRY
dc.contributor.departmentPATHOLOGY
dc.contributor.departmentDUKE-NUS GRADUATE MEDICAL SCHOOL S'PORE
dc.description.doi10.1101/gr.113555.110
dc.description.sourcetitleGenome Research
dc.description.volume21
dc.description.issue5
dc.description.page665-675
dc.description.codenGEREF
dc.identifier.isiut000290088000004
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.