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|Title:||Chromatin interaction analysis with Paired-End Tag Sequencing (ChIA-PET) for mapping chromatin Interactions and Understanding Transcription Regulation||Authors:||Goh, Y.
|Issue Date:||30-Apr-2012||Citation:||Goh, Y., Fullwood, M.J., Poh, H.M., Peh, S.Q., Ong, C.T., Zhang, J., Ruan, X., Ruan, Y. (2012-04-30). Chromatin interaction analysis with Paired-End Tag Sequencing (ChIA-PET) for mapping chromatin Interactions and Understanding Transcription Regulation. Journal of Visualized Experiments (62) : -. ScholarBank@NUS Repository. https://doi.org/10.3791/3770||Abstract:||Genomes are organized into three-dimensional structures, adopting higher-order conformations inside the micron-sized nuclear spaces. Such architectures are not random and involve interactions between gene promoters and regulatory elements. The binding of transcription factors to specific regulatory sequences brings about a network of transcription regulation and coordination. Chromatin Interaction Analysis by Paired-End Tag Sequencing (ChIA-PET) was developed to identify these higher-order chromatin structures. Cells are fixed and interacting loci are captured by covalent DNA-protein cross-links. To minimize non-specific noise and reduce complexity, as well as to increase the specificity of the chromatin interaction analysis, chromatin immunoprecipitation (ChIP) is used against specific protein factors to enrich chromatin fragments of interest before proximity ligation. Ligation involving half-linkers subsequently forms covalent links between pairs of DNA fragments tethered together within individual chromatin complexes. The flanking MmeI restriction enzyme sites in the half-linkers allow extraction of paired end tag-linker-tag constructs (PETs) upon MmeI digestion. As the half-linkers are biotinylated, these PET constructs are purified using streptavidin-magnetic beads. The purified PETs are ligated with next-generation sequencing adaptors and a catalog of interacting fragments is generated via next-generation sequencers such as the Illumina Genome Analyzer. Mapping and bioinformatics analysis is then performed to identify ChIP-enriched binding sites and ChIP-enriched chromatin interactions. We have produced a video to demonstrate critical aspects of the ChIA-PET protocol, especially the preparation of ChIP as the quality of ChIP plays a major role in the outcome of a ChIA-PET library. As the protocols are very long, only the critical steps are shown in the video. © 2012 Creative Commons Attribution License.||Source Title:||Journal of Visualized Experiments||URI:||http://scholarbank.nus.edu.sg/handle/10635/109241||ISSN:||1940087X||DOI:||10.3791/3770|
|Appears in Collections:||Staff Publications|
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