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Title: Cdc28-Cln3 phosphorylation of Sla1 regulates actin patch dynamics in different modes of fungal growth
Authors: Zeng, G.
Wang, Y.-M.
Wang, Y. 
Issue Date: 1-Sep-2012
Citation: Zeng, G., Wang, Y.-M., Wang, Y. (2012-09-01). Cdc28-Cln3 phosphorylation of Sla1 regulates actin patch dynamics in different modes of fungal growth. Molecular Biology of the Cell 23 (17) : 3485-3497. ScholarBank@NUS Repository.
Abstract: A dynamic balance between targeted transport and endocytosis is critical for polarized cell growth. However, how actin-mediated endocytosis is regulated in different growth modes remains unclear. Here we report differential regulation of cortical actin patch dynamics between the yeast and hyphal growth in Candida albicans. The mechanism involves phosphoregulation of the endocytic protein Sla1 by the cyclin-dependent kinase (CDK) Cdc28-Cln3 and the actin-regulating kinase Prk1. Mutational studies of the CDK phosphorylation sites of Sla1 revealed that Cdc28-Cln3 phosphorylation of Sla1 enhances its further phosphorylation by Prk1, weakening Sla1 association with Pan1, an activator of the actin-nucleating Arp2/3 complex. Sla1 is rapidly dephosphorylated upon hyphal induction and remains so throughout hyphal growth. Consistently, cells expressing a phosphomimetic version of Sla1 exhibited markedly reduced actin patch dynamics, impaired endocytosis, and defective hyphal development, whereas a nonphosphorylatable Sla1 had the opposite effect. Taken together, our findings establish a molecular link between CDK and a key component of the endocytic machinery, revealing a novel mechanism by which endocytosis contributes to cell morphogenesis. © 2012 Zeng et al.
Source Title: Molecular Biology of the Cell
ISSN: 10591524
DOI: 10.1091/mbc.E12-03-0231
Appears in Collections:Staff Publications

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