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Title: AMP kinase activation mitigates dopaminergic dysfunction and mitochondrial abnormalities in Drosophila models of Parkinson's disease
Authors: Ng, C.-H.
Guan, M.S.H.
Koh, C.
Ouyang, X.
Yu, F.
Tan, E.-K.
O'Neill, S.P.
Zhang, X.
Chung, J.
Lim, K.-L. 
Issue Date: 10-Oct-2012
Citation: Ng, C.-H., Guan, M.S.H., Koh, C., Ouyang, X., Yu, F., Tan, E.-K., O'Neill, S.P., Zhang, X., Chung, J., Lim, K.-L. (2012-10-10). AMP kinase activation mitigates dopaminergic dysfunction and mitochondrial abnormalities in Drosophila models of Parkinson's disease. Journal of Neuroscience 32 (41) : 14311-14317. ScholarBank@NUS Repository.
Abstract: Mutations in parkin and LRRK2 together account for the majority of familial Parkinson's disease (PD) cases. Interestingly, recent evidence implicates the involvement ofparkin and LRRK2inmitochondrial homeostasis. Supporting this, we show here bymeansof the Drosophila model system that, like parkin, LRRK2 mutations induce mitochondrial pathology in flies when expressed in their flight muscles, the toxic effects of which can be rescued by parkin coexpression. When expressed specifically in fly dopaminergic neurons, mutant LRRK2 results in the appearance of significantly enlarged mitochondria, a phenotype that can also be rescued by parkin coex-pression. Importantly, we also identified in this study that epigallocatechin gallate (EGCG), a green tea-derived catechin, acts as a potent suppressor of dopaminergic and mitochondrial dysfunction in both mutant LRRK2 and parkin-null flies. Notably, the protective effects of EGCG are abolished when AMP-activated protein kinase (AMPK)isgeneticallyinactivated, suggesting that EGCG-mediated neuropro-tection requiresAMPK.Consistent with this,direct pharmacologicalorgenetic activationofAMPKreproduces EGCG'sprotective effects. Conversely, loss of AMPK activity exacerbates neuronal loss and associated phenotypes in parkin and LRRK mutant flies. Together, our results suggestthe relevanceofmitochondrial-associatedpathwayinLRRK2 and parkin-related pathogenesis, and that AMPK activation may represent a potential therapeutic strategy for these familial forms of PD. © 2012 the authors.
Source Title: Journal of Neuroscience
ISSN: 02706474
DOI: 10.1523/JNEUROSCI.0499-12.2012
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