Please use this identifier to cite or link to this item:
https://doi.org/10.1007/978-1-61779-573-2_13
DC Field | Value | |
---|---|---|
dc.title | 2D DIGE analysis of serum after fractionation by proteominer™ beads | |
dc.contributor.author | Liang, C. | |
dc.contributor.author | Tan, G.S. | |
dc.contributor.author | Chung, M.C.M. | |
dc.date.accessioned | 2014-11-26T07:42:15Z | |
dc.date.available | 2014-11-26T07:42:15Z | |
dc.date.issued | 2012 | |
dc.identifier.citation | Liang, C.,Tan, G.S.,Chung, M.C.M. (2012). 2D DIGE analysis of serum after fractionation by proteominer™ beads. Methods in Molecular Biology 854 : 181-194. ScholarBank@NUS Repository. <a href="https://doi.org/10.1007/978-1-61779-573-2_13" target="_blank">https://doi.org/10.1007/978-1-61779-573-2_13</a> | |
dc.identifier.isbn | 9781617795725 | |
dc.identifier.issn | 10643745 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/109134 | |
dc.description.abstract | Serum is a popular biofluid used for many protein biomarker discovery projects since the collection and processing of serum/plasma is relatively noninvasive and inexpensive. Unfortunately, the downstream analysis of serum/plasma is hampered severely by several high-abundant proteins which often interfere with the separation and detection of many of the proteins of lower abundance. Thus, a number of prefractionation methods have recently been developed with the view to reduce the dynamic range of these proteins. These include both dye- and immunoaffinity-based methods that are specifically designed to remove serum albumin. In this chapter, we describe an alternative method using ProteoMiner™ or Equalizer beads that is aimed at overcoming this problem in serum. This method uses a combinatorial library of hexapeptides bound to beads and works by binding proteins until saturation is reached. Thus, the high-abundant proteins will reach saturation quickly, while the lower-abundant proteins continue to bind. This results in a dramatic depletion of the most abundant proteins, with a concurrent concentration of the middle- to low-abundant proteins. © 2012 Springer Science+Business Media, LLC. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/978-1-61779-573-2_13 | |
dc.source | Scopus | |
dc.subject | 2D DIGE | |
dc.subject | Equalizer beads | |
dc.subject | ProteoMiner™ | |
dc.subject | Serum | |
dc.type | Article | |
dc.contributor.department | MECHANOBIOLOGY INSTITUTE | |
dc.contributor.department | BIOCHEMISTRY | |
dc.description.doi | 10.1007/978-1-61779-573-2_13 | |
dc.description.sourcetitle | Methods in Molecular Biology | |
dc.description.volume | 854 | |
dc.description.page | 181-194 | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Staff Publications |
Show simple item record
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.