Please use this identifier to cite or link to this item: https://doi.org/10.1083/jcb.201003005
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dc.titlep125A exists as part of the mammalian Sec13/Sec31 COPII subcomplex to facilitate ER-Golgi transport
dc.contributor.authorOng, Y.S.
dc.contributor.authorTang, B.L.
dc.contributor.authorLoo, L.S.
dc.contributor.authorHong, W.
dc.date.accessioned2014-11-25T09:46:42Z
dc.date.available2014-11-25T09:46:42Z
dc.date.issued2010-08-09
dc.identifier.citationOng, Y.S., Tang, B.L., Loo, L.S., Hong, W. (2010-08-09). p125A exists as part of the mammalian Sec13/Sec31 COPII subcomplex to facilitate ER-Golgi transport. Journal of Cell Biology 190 (3) : 331-345. ScholarBank@NUS Repository. https://doi.org/10.1083/jcb.201003005
dc.identifier.issn00219525
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/108484
dc.description.abstractCoat protein II (COPII)-mediated export from the endoplasmic reticulum (ER) involves sequential recruitment of COPII complex components, including the Sar1 GTPase, the Sec23/Sec24 subcomplex, and the Sec13/Sec31 subcomplex. p125A was originally identified as a Sec23A-interacting protein. Here we demonstrate that p125A also interacts with the C-terminal region of Sec31A. The Sec31A-interacting domain of p125A is between residues 260-600, and is therefore a distinct domain from that required for interaction with Sec23A. Gel filtration and immunodepletion studies suggest that the majority of cytosolic p125A exists as a ternary complex with the Sec13/Sec31A subcomplex, suggesting that Sec 13, Sec31A, and p125A exist in the cytosol primarily as preassembled Sec13/Sec31A/ p125A heterohexamers. Golgi morphology and protein export from the ER were affected in p125A-silenced cells. Our results suggest that p125A is part of the Sec13/ Sec31A subcomplex and facilitates ER export in mammalian cells. © 2010 Ong et al.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1083/jcb.201003005
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOCHEMISTRY
dc.description.doi10.1083/jcb.201003005
dc.description.sourcetitleJournal of Cell Biology
dc.description.volume190
dc.description.issue3
dc.description.page331-345
dc.description.codenJCLBA
dc.identifier.isiut000280691000007
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