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Title: Noninvasive urinary metabonomic diagnosis of human bladder cancer
Authors: Pasikanti, K.K.
Esuvaranathan, K.
Ho, P.C. 
Mahendran, R. 
Kamaraj, R.
Wu, Q.H.
Chiong, E.
Chan, E.C.Y. 
Keywords: bladder cancer
gas chromatography mass spectrometry
metabolic profiling
Issue Date: 4-Jun-2010
Citation: Pasikanti, K.K., Esuvaranathan, K., Ho, P.C., Mahendran, R., Kamaraj, R., Wu, Q.H., Chiong, E., Chan, E.C.Y. (2010-06-04). Noninvasive urinary metabonomic diagnosis of human bladder cancer. Journal of Proteome Research 9 (6) : 2988-2995. ScholarBank@NUS Repository.
Abstract: Cystoscopy is considered the gold standard for the clinical diagnosis of human bladder cancer (BC). As cystoscopy is expensive and invasive, it may compromise patients' compliance and account for the failure in detecting recurrent BC in some patients. In this paper, we investigated the role of urinary metabonomics in the diagnosis of human BC. Gas chromatography/time-of- flight mass spectrometry was applied for the urinary metabolic profiling of 24 BC patients and 51 non-BC controls. The acquired data were analyzed using multivariate principal component analysis followed by orthogonal partial least-squares discriminant analysis (OPLS-DA). Model validity was verified using permutation tests and receiver operating characteristic (ROC) analysis. BC patients were clearly distinguished from non-BC subjects based on their global urinary metabolic profiles (OPLS-DA, 4 latent variables, R2X) 0.420, R2Y) 0.912 and Q2 (cumulative)) 0.245; ROC AUC of 0.90; 15 marker metabolites). One-hundred percent sensitivity in detecting BC was observed using urinary metabonomics versus 33% sensitivity achieved by urinary cytology. Additionally, urinary metabonomics exhibited potential in the staging and grading of bladder tumors. In summary, urinary metabonomics is amenable for the noninvasive diagnosis of human BC. © 2010 American Chemical Society.
Source Title: Journal of Proteome Research
ISSN: 15353893
DOI: 10.1021/pr901173v
Appears in Collections:Staff Publications

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