Please use this identifier to cite or link to this item:
|Title:||mGluR5-PLCβ4-PKCβ2/PKCγ pathways in hippocampal CA1 pyramidal neurons in pilocarpine model of status epilepticus in mGluR5+/+ mice||Authors:||Liu, J.X.
Metabotropic glutamate receptor 5
Protein kinase C
|Issue Date:||Dec-2008||Citation:||Liu, J.X., Tang, Y.C., Liu, Y., Tang, F.R. (2008-12). mGluR5-PLCβ4-PKCβ2/PKCγ pathways in hippocampal CA1 pyramidal neurons in pilocarpine model of status epilepticus in mGluR5+/+ mice. Epilepsy Research 82 (2-3) : 111-123. ScholarBank@NUS Repository. https://doi.org/10.1016/j.eplepsyres.2008.07.014||Abstract:||While it is generally accepted that phospholipase C (PLC) and protein kinase C (PKC) are down-stream proteins involved in metabotropic glutamate receptor 5 (mGluR5)-related signal transduction, we still do not know which subtype of PLC or PKC is specifically regulated after mGluR5 activation. In the present study in mGluR5 wild-type (mGluR5+/+) mice, we showed induced PKCβ2 or PKCγ expression at the border between the stratum oriens and alveus (O/A border) at 2 h during pilocarpine induced status epilepticus (SE), and in the stratum pyramidale in CA1 area at 1 day after pilocarpine induced SE; at 1 day, induced expression of PLCβ4 in the stratum pyramidale of CA1 area was observed. Furthermore, double labeling revealed the co-localization of induced PKCβ2 or PKCγ with mGluR5 or with induced PLCβ4 in the stratum pyramidale of CA1 area. These induced expression, however, were not found in mGluR5 mutant (mGluR5-/-) mice. It suggests that induced PLCβ4-PKCβ2/PKCγ at 1 day after pilocarpine induced SE in pyramidal neurons or PKCβ2 or PKCγ in interneurons at O/A border at 2 h during pilocarpine induced SE may be specifically linked to the activation of mGluR5. When compared to mGluR5+/+ mice, significant shorter latency (from pilocarpine injection to the occurrence of status epilepticus) and maintenance period (from beginning to the end of status epilepticus) for status epilepticus in mGluR5-/- mice were also demonstrated. It is possible that mGluR5 may play a negative role in initiation of status epilepticus by interacting with muscarinic acetylcholine receptor in mGluR5+/+ mice. © 2008 Elsevier B.V. All rights reserved.||Source Title:||Epilepsy Research||URI:||http://scholarbank.nus.edu.sg/handle/10635/108465||ISSN:||09201211||DOI:||10.1016/j.eplepsyres.2008.07.014|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Feb 24, 2020
WEB OF SCIENCETM
checked on Feb 24, 2020
checked on Feb 22, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.