Please use this identifier to cite or link to this item: https://doi.org/10.1111/j.1582-4934.2009.00779.x
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dc.titleHepatoma-derived growth factor and its role in keloid pathogenesis
dc.contributor.authorOoi, B.N.S.
dc.contributor.authorMukhopadhyay, A.
dc.contributor.authorMasilamani, J.
dc.contributor.authorDo, D.V.
dc.contributor.authorLim, C.P.
dc.contributor.authorCao, X.M.
dc.contributor.authorLim, I.J.
dc.contributor.authorMao, L.
dc.contributor.authorRen, H.N.
dc.contributor.authorNakamura, H.
dc.contributor.authorPhan, T.T.
dc.date.accessioned2014-11-25T09:45:44Z
dc.date.available2014-11-25T09:45:44Z
dc.date.issued2010-06
dc.identifier.citationOoi, B.N.S., Mukhopadhyay, A., Masilamani, J., Do, D.V., Lim, C.P., Cao, X.M., Lim, I.J., Mao, L., Ren, H.N., Nakamura, H., Phan, T.T. (2010-06). Hepatoma-derived growth factor and its role in keloid pathogenesis. Journal of Cellular and Molecular Medicine 14 (6 A) : 1328-1337. ScholarBank@NUS Repository. https://doi.org/10.1111/j.1582-4934.2009.00779.x
dc.identifier.issn15821838
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/108399
dc.description.abstractHepatoma-derived growth factor (HDGF) is a novel mitogenic growth factor that has been implicated in many different carcinomas. Its role in keloid biology has not yet been investigated. The present study is aimed at examining the role of HDGF in keloid pathogenesis. Immunohistochemical staining and Western blot analyses were used to examine in vivo localization and expression of HDGF in keloid and normal skin tissue. This was followed by the detection of HDGF expression in fibroblasts cultured in vitro and fibroblasts exposed to serum. To investigate the effect of epithelial-mesenchymal interactions, a two-chamber system was employed in which keratinocytes on membrane inserts were co-cultured with the fibroblasts. HDGF expression levels in all cell extracts and conditioned media were assayed through Western blot analysis. In another set of experiments, the effect of exogenous recombinant HDGF on keloid fibroblasts (KF) and normal fibroblasts (NF) was examined. Cell proliferation was assessed by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and by quantifying proliferating cell nuclear antigen (PCNA) expression. Downstream targets of HDGF were identified by detecting their expression through Western blot analysis. Our results indicate that there was an increase in HDGF expression in the dermis of keloid compared with normal skin tissue. The application of serum and epithelial-mesenchymal interactions did not seem to have any effect on intracellular HDGF expression levels. However, co-culturing keloid keratinocytes with KFs resulted in increased HDGF secretion when compared with monoculture or normal controls. Furthermore, treatment with exogenous recombinant HDGF was found to increase the proliferation of KFs, activate the extracellular signal-regulated kinase (ERK) pathway and up-regulate the secretion of vascular endothelial growth factor (VEGF). © 2009 The Authors Journal compilation © 2010 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1111/j.1582-4934.2009.00779.x
dc.sourceScopus
dc.subjectEpithelial-mesenchymal interaction
dc.subjectERK
dc.subjectHepatoma-derived growth factor
dc.subjectKeloid fibroblast
dc.subjectProliferation
dc.subjectVascular endothelial growth factor
dc.subjectWound healing
dc.typeArticle
dc.contributor.departmentSURGERY
dc.description.doi10.1111/j.1582-4934.2009.00779.x
dc.description.sourcetitleJournal of Cellular and Molecular Medicine
dc.description.volume14
dc.description.issue6 A
dc.description.page1328-1337
dc.identifier.isiut000279615500012
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