Please use this identifier to cite or link to this item:
|Title:||Establishing a toolkit for precursor-directed polyketide biosynthesis: Exploring substrate promiscuities of acid-CoA ligases||Authors:||Go, M.K.
|Issue Date:||5-Jun-2012||Citation:||Go, M.K., Chow, J.Y., Cheung, V.W.N., Lim, Y.P., Yew, W.S. (2012-06-05). Establishing a toolkit for precursor-directed polyketide biosynthesis: Exploring substrate promiscuities of acid-CoA ligases. Biochemistry 51 (22) : 4568-4579. ScholarBank@NUS Repository. https://doi.org/10.1021/bi300425j||Abstract:||Polyketides are chemically diverse and medicinally important biochemicals that are biosynthesized from acyl-CoA precursors by polyketide synthases. One of the limitations to combinatorial biosynthesis of polyketides has been the lack of a toolkit that describes the means of delivering novel acyl-CoA precursors necessary for polyketide biosynthesis. Using five acid-CoA ligases obtained from various plants and microorganisms, we biosynthesized an initial library of 79 acyl-CoA thioesters by screening each of the acid-CoA ligases against a library of 123 carboxylic acids. The library of acyl-CoA thioesters includes derivatives of cinnamyl-CoA, 3-phenylpropanoyl-CoA, benzoyl-CoA, phenylacetyl-CoA, malonyl-CoA, saturated and unsaturated aliphatic CoA thioesters, and bicyclic aromatic CoA thioesters. In our search for the biosynthetic routes of novel acyl-CoA precursors, we discovered two previously unreported malonyl-CoA derivatives (3-thiophenemalonyl-CoA and phenylmalonyl-CoA) that cannot be produced by canonical malonyl-CoA synthetases. This report highlights the utility and importance of determining substrate promiscuities beyond conventional substrate pools and describes novel enzymatic routes for the establishment of precursor-directed combinatorial polyketide biosynthesis. (Chemical Presented). © 2012 American Chemical Society.||Source Title:||Biochemistry||URI:||http://scholarbank.nus.edu.sg/handle/10635/108368||ISSN:||00062960||DOI:||10.1021/bi300425j|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jul 9, 2019
WEB OF SCIENCETM
checked on Jul 9, 2019
checked on May 31, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.