Please use this identifier to cite or link to this item:
https://doi.org/10.1210/en.2009-1418
DC Field | Value | |
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dc.title | Delineating biological pathways unique to embryonic stem cell-derived insulin-producing cell lines from their noninsulin-producing progenitor cell lines | |
dc.contributor.author | Chen, T.S. | |
dc.contributor.author | Tan, S.S. | |
dc.contributor.author | Yeo, R.W.Y. | |
dc.contributor.author | Teh, B.J. | |
dc.contributor.author | Luo, R. | |
dc.contributor.author | Li, G. | |
dc.contributor.author | Lim, S.K. | |
dc.date.accessioned | 2014-11-25T09:44:43Z | |
dc.date.available | 2014-11-25T09:44:43Z | |
dc.date.issued | 2010-08 | |
dc.identifier.citation | Chen, T.S., Tan, S.S., Yeo, R.W.Y., Teh, B.J., Luo, R., Li, G., Lim, S.K. (2010-08). Delineating biological pathways unique to embryonic stem cell-derived insulin-producing cell lines from their noninsulin-producing progenitor cell lines. Endocrinology 151 (8) : 3600-3610. ScholarBank@NUS Repository. https://doi.org/10.1210/en.2009-1418 | |
dc.identifier.issn | 00137227 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/108329 | |
dc.description.abstract | To identify unique biochemical pathways in embryonic stem cell-derived insulin-producing cells as potential therapeutic targets to prevent or delay β-cell dysfunction or death in diabetic patients, comparative genome-wide gene expression studies of recently derived mouse insulin-producing cell linesandtheir progenitor cell lines were performed using microarray technology. Differentially expressed genes were functionally clustered to identify important biochemical pathways in these insulin-producing cell lines. Biochemical or cellular assays were then performed to assess the relevance of these pathways to the biology of these cells. A total of 185 genes were highly expressed in the insulin-producing cell lines, and computational analysis predicted the pentose phosphate pathway (PPP), clathrin-mediated endocytosis, and the peroxisome proliferator-activated receptor (PPAR) signaling pathway as important pathways in these cell lines. Insulin-producing ERoSHK cells were more resistant to hydrogen peroxide (H2O2)-induced oxidative stress. Inhibition of PPP by dehydroepiandrosterone and 6-aminonicotinamide abrogated this H2O2 resistance with a concomitant decrease in PPP activity as measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Clathrin-mediated endocytosis, which is essential in maintaining membrane homeostasis in secreting cells, was up-regulated by glucose in ERoSHK but not in their progenitor ERoSH cells. Its inhibition by chlorpromazine at high glucose concentration was toxic to the cells. Troglitazone, a PPARG agonist, up-regulated expression of Ins1 and Ins2 but not Glut2. Gene expression analysis has identified the PPP, clathrin-mediated endocytosis, and the PPAR signaling pathway as the major delineating pathways in these insulin-producing cell lines, and their biological relevance was confirmed by biochemical and cellular assays. Copyright © 2010 by The Endocrine Society. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1210/en.2009-1418 | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | SURGERY | |
dc.contributor.department | NATIONAL UNIVERSITY MEDICAL INSTITUTES | |
dc.description.doi | 10.1210/en.2009-1418 | |
dc.description.sourcetitle | Endocrinology | |
dc.description.volume | 151 | |
dc.description.issue | 8 | |
dc.description.page | 3600-3610 | |
dc.description.coden | ENDOA | |
dc.identifier.isiut | 000280171100014 | |
Appears in Collections: | Staff Publications |
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