Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/108085
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dc.titleProduction of monoclonal antibodies against human hepatocellular carcinoma by immunisation with a cell membrane preparation.
dc.contributor.authorTan, L.S.
dc.date.accessioned2014-11-20T05:58:43Z
dc.date.available2014-11-20T05:58:43Z
dc.date.issued1990-03
dc.identifier.citationTan, L.S. (1990-03). Production of monoclonal antibodies against human hepatocellular carcinoma by immunisation with a cell membrane preparation.. Annals of the Academy of Medicine Singapore 19 (2) : 147-151. ScholarBank@NUS Repository.
dc.identifier.issn03044602
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/108085
dc.description.abstractMonoclonal antibodies that bound to 3 human hepatocellular carcinoma cell lines (PLC/PRF/5, SK-SF and Mahlavu) and snap-frozen sections of a patient's tumour were raised by immunising mice with PLC/PRF/5 membrane preparations. The monoclonals designated RF-HCC 1 and RF-HCC 2 were of IgG1 and IgM subclasses respectively. Binding of the antibodies to surface epitopes present on Mahlavu cells in vitro took place in a biphasic fashion, with initial rapid binding of each antibody during the first 15 minutes of incubation followed by a plateau after this period. It was found that the commercially available MOPC 21 myeloma IgG1 (Sigma) monoclonal antibody used a control also bound to a surface antigenic determinant expressed on Mahlavu cells.
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentPHARMACOLOGY
dc.description.sourcetitleAnnals of the Academy of Medicine Singapore
dc.description.volume19
dc.description.issue2
dc.description.page147-151
dc.identifier.isiutNOT_IN_WOS
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